Effects and possible mechanisms of Alpinia officinarum ethanol extract on indomethacin-induced gastric injury in rats.
Effects and possible mechanisms of Alpinia officinarum ethanol extract on indomethacin-induced gastric injury in rats.
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高良姜乙醇提取物对吲哚美辛所致大鼠胃损伤的影响及可能机制
DOI：
10.1080/13880209.2018.1450426
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发表时间：
2018-12
影响因子：
3.8
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Gong J;Zhang Z;Zhang X;Chen F;Tan Y;Li H;Jiang J;Zhang J
抽象上下文：高良姜（Alpinia officinarum Hance）在治疗胃肠道疾病方面具有悠久的历史，但其作用机制尚不清楚。目的：探讨黄芪醇提物的抗肿瘤作用及其机制。在吲哚美辛诱导的胃损伤大鼠模型中，材料和方法：向Sprague-Dawley大鼠口服给予吲哚美辛（0.3g/kg）以诱导胃损伤; 7小时后，用0.03、0.09或0.18g/kg的植物提取物、高良姜素（0.2g/kg）或柠檬酸铋钾（0.08g/kg）处理大鼠，每天一次，持续6天。对照组大鼠接受等体积溶剂溶液6天。以大体溃疡和组织学指标评价胃损伤。通过蛋白质印迹和ELISA定量环氧合酶和非环氧合酶途径蛋白。结果如下：高良姜提取物以剂量依赖性方式减轻胃损伤，并且0.18g/kg剂量通过减少大体溃疡表现出最佳性能（从20.23 ± 1.38到1.66 ± 0.37）和组织学（从4.67 ± 1.03降至0.33 ± 0.51）指数，降低血清TNF-α水平（14.17%），血清VEGF水平升高（1.58倍），增加环氧合酶-1水平（1.25倍，P < 0.001），逆转吲哚美辛诱导的非环氧化酶途径蛋白表达的变化（p < 0.05）。高良姜素作为抗溃疡剂的有效性低于整个提取物，表明其他成分也有助于保护作用。结论：高良姜提取物和高良姜素通过环氧合酶和非环氧合酶途径发挥抗溃疡作用，验证高良姜素作为胃损伤治疗的用途。
Abstract Context: Alpinia officinarum Hance (Zingiberoside) has a long history in treating gastrointestinal diseases, but its mechanisms of action are not yet known. Objective: To investigate the effects and underlying mechanisms of the ethanol extract of A. officinarum rhizomes in an indomethacin-induced gastric injury rat model. Material and methods: Indomethacin (0.3 g/kg) was orally administered to Sprague-Dawley rats to induce gastric damage; after 7 h, the rats were treated with 0.03, 0.09, or 0.18 g/kg of the plant extract, galangin (0.2 g/kg), or bismuth potassium citrate (0.08 g/kg), once a day for 6 days. Rats in the control group received an equivalent volume of vehicle solution for 6 days. Gastric damage was evaluated by gross ulcer and histological indexes. Cyclooxygenase and non-cyclooxygenase pathway proteins were quantified by western blotting and ELISA. Results: Alpinia officinarum extract ameliorated gastric injury in a dose-dependent manner, and 0.18 g/kg dose exhibited the best performance by reducing the gross ulcer (from 20.23 ± 1.38 to 1.66 ± 0.37) and histological (from 4.67 ± 1.03 to 0.33 ± 0.51) indexes, decreasing serum TNF-α level (14.17%), increasing serum VEGF level (1.58 times), increasing cyclooxygenase-1 level (1.25 times, p < 0.001) in the gastric mucosa, and reversing indomethacin-induced changes in the expression of non-cyclooxygenase pathway proteins (p < 0.05). Galangin was less effective as an antiulcer agent than the whole extract, indicating that other components also contributed to the protective effect. Conclusions: Alpinia officinarum extract and galangin exert antiulcer effects through cyclooxygenase and non-cyclooxygenase pathways validating use of galangin as a treatment for gastric damage.
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影响因子：
6.1
作者：
Gao, YH;Zhou, SF;Xu, AL
通讯作者：
Xu, AL
影响因子：
29.4
作者：
Lanas, Angel;Baron, John A.;Bresalier, Robert
通讯作者：
Bresalier, Robert
影响因子：
9
作者：
GANA, TJ;HUHLEWYCH, R;KOO, J
通讯作者：
KOO, J
影响因子：
29.4
作者：
Laine, L
通讯作者：
Laine, L
影响因子：
7.6
作者：
Abraham, N. S.;El-Serag, H. B.;Deswal, A.
通讯作者：
Deswal, A.