Fluorination of mammalian cell surfaces via the sialic acid biosynthetic pathway.
Fluorination of mammalian cell surfaces via the sialic acid biosynthetic pathway.
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DOI：
10.1016/j.bmcl.2008.09.010
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发表时间：
2008-11-15
影响因子：
2.7
通讯作者：
Kumar K
中科院分区：
文献类型：
--
作者：
Dafik L;d'Alarcao M;Kumar K
Metabolic oligosaccharide engineering has been employed to introduce fluorine-containing groups onto mammalian cell surfaces. Incubation of HeLa, Jurkat, and HL60 cells in culture with fluorinated sialic acid and mannosamine analogues resulted in cell-surface presentation of fluorinated glycans. Metabolic conversion of fluorinated precursors was detected and quantified by DMB-derivatization and HPLC ESI-MS analysis. Between 7% and 72% of total membrane-associated sialosides were fluorinated, depending on the precursor used and the cell type. Fluorination of mammalian cell surfaces provides a means for introducing a bioorthogonal surface for modulating noncovalent interactions such as those involved in cell adhesion.
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影响因子：
2.9
作者：
HARA, S;TAKEMORI, Y;OHKURA, Y
通讯作者：
OHKURA, Y
DOI：
10.1006/bbrc.1998.9650
发表时间：
1998-12-18
影响因子：
3.1
作者：
Keppler, OT;Herrmann, M;Pawlita, M
通讯作者：
Pawlita, M
影响因子：
4.7
作者：
Villavicencio-Lorini, P;Laabs, S;Horstkorte, R
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Horstkorte, R
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3.2
作者：
Luchansky, SJ;Goon, S;Bertozzi, CR
通讯作者：
Bertozzi, CR
影响因子：
4.8
作者：
Oetke, C;Brossmer, R;Pawlita, M
通讯作者：
Pawlita, M