Metrics for assessing cytoskeletal orientational correlations and consistency.
Metrics for assessing cytoskeletal orientational correlations and consistency.
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DOI：
10.1371/journal.pcbi.1004190
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发表时间：
2015-04
影响因子：
4.3
通讯作者：
Grosberg A
中科院分区：
文献类型：
--
作者：
Drew NK;Eagleson MA;Baldo DB Jr;Parker KK;Grosberg A
在生物学中，多尺度的组织增强了生物功能。当前生物组织染色和成像技术的进步提供了大量数据，但很少有指标能定量描述这些发现。尤其需要一种能表征组织内不同生物结构取向的相关性和一致性的指标。我们旨在创建这样一种指标，并展示其在心脏组织图像中的应用。共取向序参数（COOP）基于经典参数取向序参数（OOP）的数学框架。相关定理被证明以阐明COOP的性质和边界，然后将其应用于合成数据和实验数据。我们表明COOP可用于量化诸如肌动蛋白丝和肌节Z线等结构取向的相关性。正如预期的那样，心脏组织中肌动蛋白丝和Z线之间呈现出完美的相关性。我们还展示了使用COOP来量化相同形状细胞内结构取向的一致性。COOP提供了一种定量工具，用于表征超出共定位或单一结构取向分布的组织。未来，这一新参数可用于表示心脏组织成熟过程、病理畸形及其他过程中的定量变化。
生物组织在多个长度尺度上具有高度的组织性。在组织工程中，重现体内结构是体外实验的一个重要方面。能够量化细胞结构的组织性，特别是其取向的相关性，将极大地增进我们对每种结构功能的理解，并为评估工程组织提供一种工具。在这项工作中，我们开发了一个参数来评估结构取向的相关性和一致性。我们对这个共取向序参数进行了广泛的分析表征，使用合成数据对其进行了验证，并展示了其在工程心脏组织中Z线和肌动蛋白纤维结构的实验数据中的应用。只要知道结构的取向角度和位置，该参数就能量化取向相关性和一致性。因此，共取向序参数在生物学及潜在的其他领域都有广泛的应用范围。
In biology, organization at multiple scales potentiates biological function. Current advances in staining and imaging of biological tissues provide a wealth of data, but there are few metrics to quantitatively describe these findings. In particular there is a need for a metric that would characterize the correlation and consistency of orientation of different biological constructs within a tissue. We aimed to create such a metric and to demonstrate its use with images of cardiac tissues. The co-orientational order parameter (COOP) was based on the mathematical framework of a classical parameter, the orientational order parameter (OOP). Theorems were proven to illustrate the properties and boundaries of the COOP, which was then applied to both synthetic and experimental data. We showed the COOP to be useful for quantifying the correlation of orientation of constructs such as actin filaments and sarcomeric Z-lines. As expected, cardiac tissues showed perfect correlation between actin filaments and Z-lines. We also demonstrated the use of COOP to quantify the consistency of construct orientation within cells of the same shape. The COOP provides a quantitative tool to characterize tissues beyond co-localization or single construct orientation distribution. In the future, this new parameter could be used to represent the quantitative changes during maturation of cardiac tissue, pathological malformation, and other processes. Biological tissues are highly organized on multiple length scales. In tissue engineering, recreating the in vivo architecture is an important aspect of in vitro experiments. An ability to quantify organization of cellular constructs, specifically the correlation in their orientations, would greatly enhance both our understanding of the function of each construct and provide a tool to evaluate engineered tissues. In this work, we have developed a parameter to evaluate the correlation and consistency of construct orientation. We have extensively characterized this co-orientational order parameter analytically, validated it using synthetic data, and demonstrated its use with experimental data of Z-lines and actin fibrils architectures in engineered cardiac tissues. As long as the orientation angles and location of constructs are known, the parameter can quantify both orientational correlation and consistency. Thus, the co-orientational order parameter has a wide scope of application both in biology and potentially outside of it.
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影响因子：
37.8
作者：
Aikawa, E;Whittaker, P;Schoen, FJ
通讯作者：
Schoen, FJ
影响因子：
14
作者：
Feinberg, Adam W.;Alford, Patrick W.;Jin, Hongwei;Ripplinger, Crystal M.;Werdich, Andreas A.;Sheehy, Sean P.;Grosberg, Anna;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
6.1
作者：
Grosberg A;Alford PW;McCain ML;Parker KK
通讯作者：
Parker KK
DOI：
10.1109/34.709565
发表时间：
1998-08-01
影响因子：
23.6
作者：
Hong, L;Wan, YF;Jain, A
通讯作者：
Jain, A
影响因子：
9.7
作者：
Davidson, P.;Bigerelle, M.;Anselme, K.
通讯作者：
Anselme, K.