Association between the dynamics of multiple replication origins and the evolution of multireplicon genome architecture in haloarchaea.
Association between the dynamics of multiple replication origins and the evolution of multireplicon genome architecture in haloarchaea.
复制标题
盐古菌多复制起点动态与多复制子基因组结构进化之间的关联
DOI：
10.1093/gbe/evu219
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发表时间：
2014-10-03
影响因子：
3.3
通讯作者：
Xiang H
中科院分区：
文献类型：
--
作者：
Wu Z;Yang H;Liu J;Wang L;Xiang H
古生菌基因组一般由多个复制子组成，每个复制子有一个或多个复制起点(S)。对近缘物种复制起源的比较基因组分析可以用来揭示解释多起源系统发展的进化机制。多个复制起始点已经在硅胶和实验中被研究了，这为比较多个复制起始点提供了可能性。因此，我们对Hispanica复制起源与另外五个Haloarcula物种的复制起源进行了比较。我们证明，染色体中的多个复制起点是从依赖oriC1的祖先染色体多次独立进化而来的。其中，oriC1和oriC2两个起始点在位置上是保守的，并且它们都与rRNA操纵子相邻，提示了可能促进这两个起始点保守的周边基因的复制和表达的相关性。一些染色体可变区被用作起源进化的热点，其中复制起点不断地被获得、丢失和破坏。此外，我们还证明了以H.hispanica微小染色体复制起点自主复制序列质粒是非常不稳定的。由于微染色体的组织起源和复制起源都不保守，我们提出了染色体外复制子的进化与起源变异之间的关联。综上所述，我们提供了对海洋生物多个复制起始点的进化史的见解，并提出了一个多复制起始点的动态与海洋古生菌多复制子基因组结构进化之间的关联的一般模型。
Haloarchaeal genomes are generally composed of multiple replicons, and each replicon has a single or multiple replication origin(s). The comparative genomic analysis of replication origins from closely related species can be used to reveal the evolutionary mechanisms that account for the development of multiple origin systems. Multiple replication origins have been in silico and experimentally investigated in Haloarcula hispanica, which raise the possibility for comparisons of multiple replication origins in Haloarcula species. Thus, we performed a comparison of H. hispanica replication origins with those from five additional Haloarcula species. We demonstrated that the multiple replication origins in the chromosome were evolved independently multiple times from the oriC1-dependent ancestral chromosome. Particularly, the two origins oriC1 and oriC2 were conserved in location, and both of them were adjacent to an rRNA operon, suggestive of correlations in replication and expression of surrounding genes that may promote the conservation of these two origins. Some chromosomal variable regions were used as hotspots for origin evolution in which replication origins were continually being acquired, lost, and disrupted. Furthermore, we demonstrated that autonomously replicating sequence plasmids with H. hispanica minichromosomal replication origins were extremely unstable. Because both organization and replication origins of minichromosomes were not conserved, we proposed an association between the evolution of extrachromosomal replicons and origin variation. Taken together, we provided insights into the evolutionary history of multiple replication origins in Haloarcula species, and proposed a general model of association between the dynamics of multiple replication origins and the evolution of multireplicon genome architecture in haloarchaea.
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影响因子：
3.2
作者：
Berquist, BR;DasSarma, S
通讯作者：
DasSarma, S
西班牙盐弧菌的完整基因组序列，用于研究遗传学、代谢和病毒-宿主相互作用的盐古菌模型
影响因子：
3.2
作者：
Liu, Hailong;Wu, Zhenfang;Xiang, Hua
通讯作者：
Xiang, Hua
DOI：
10.1073/pnas.0806414105
发表时间：
2008-10-28
影响因子：
11.1
作者：
Duggin, Iain G.;McCallum, Simon A.;Bell, Stephen D.
通讯作者：
Bell, Stephen D.
影响因子：
56.9
作者：
Dueber, Erin L. Cunningham;Corn, Jacob E.;Berger, James M.
通讯作者：
Berger, James M.
影响因子：
56.9
作者：
Myllykallio, H;Lopez, P;Forterre, P
通讯作者：
Forterre, P