Non-contact trapping and stretching of biological cells using dual-beam optical stretcher on microfluidic platform
Non-contact trapping and stretching of biological cells using dual-beam optical stretcher on microfluidic platform
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微流控平台上双光束光学拉伸器对生物细胞的非接触捕获和拉伸
DOI：
10.1117/12.2514299
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Su, Zhongqing
中科院分区：
文献类型：
--
作者：
Dong, Aotuo;Uppalapati, Balaadithya;Islam, Md. Shariful;Gibbs, Brandon;Kamatchi, Ganesan;Albin, Sacharia;Deo, Makarand;Fromme, Paul;Su, Zhongqing
光学拉伸器是一种工具，其中使用两束反向传播、稍微发散且相同的激光束来捕获并轴向拉伸光路中的微粒。在这项工作中，我们利用双光束光学拉伸器装置来捕获和拉伸人类胚胎肾（HEK）细胞和哺乳动物乳腺癌（MBC）细胞。实验通过将 HEK 细胞暴露于功率范围为 100 mW 至 561 mW 的反向传播激光束 30 秒来进行。据观察，电池变形的百分比从 100 mW 光功率时的 16.7% 增加到 561 mW 光功率时的 40.5%。在相同功率（80 mW）下，与 HEK 细胞相比，MBC 细胞表现出明显更高的细胞拉伸。此外，HEK 细胞的最小捕获功率为 80.5mW，而 MBC 细胞的最小捕获功率为 65.2mW。这项研究为基于非接触光学细胞拉伸的不同细胞类型的细胞骨架弹性表征提供了有用的见解。
Optical stretcher is a tool in which two counter-propagating, slightly diverging, and identical laser beams are used to trap and axially stretch microparticles in the path of light. In this work, we utilized the dual-beam optical stretcher setup to trap and stretch human embryonic kidney (HEK) cells and mammalian breast cancer (MBC) cells. Experiments were performed by exposing the HEK cells to counter-propagating laser beams for 30 seconds at powers ranging from 100 mW to 561 mW. It was observed that the percentage of cell deformation increased from 16.7% at 100 mW to 40.5% at 561 mW optical power. The MBC cells exhibited significantly higher cell stretching compared to HEK cells at the same power (80 mW). Moreover, the minimum trapping power in HEK cells was 80.5mW as compared to 65.2mW in MBC cells. This study provides useful insights into the characterization of cytoskeletal elasticity in different cell types based on non-contact optical cell stretching.
光学展宽器中球形单元表面的局部散射应力分布。
影响因子：
3.8
作者：
Paul B Bareil;Y. Sheng;A. Chiou
通讯作者：
A. Chiou
影响因子：
3.8
作者：
Bareil, Paul B.;Sheng, Yunlong;Chiou, Arthur
通讯作者：
Chiou, Arthur
微孔板：一种用于单个细胞操纵和机械扰动的新工具。
影响因子：
--
作者：
O. Thoumine;A. Ott;O. Cardoso;J. Meister
通讯作者：
J. Meister