Replication of human cytomegalovirus in cells deficient in beta 2-microglobulin gene expression.
Replication of human cytomegalovirus in cells deficient in beta 2-microglobulin gene expression.
复制标题
人类巨细胞病毒在β2-微球蛋白基因表达缺陷的细胞中的复制。
DOI：
10.1099/0022-1317-75-10-2755
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Shanley,JD
中科院分区：
文献类型：
--
作者：
Wu,QH;Trymbulak,W;Tatake,RJ;Forman,SJ;Zeff,RA;Shanley,JD
为研究β2-微球蛋白（β2-m）和主要组织相容性复合体（MHC）Ⅰ类分子表达在人巨细胞病毒（HCMV）感染中的作用，检测了HCMV AD-169株感染人黑色素瘤细胞系（FO-1）的能力。将FO-1细胞的易感性与人包皮成纤维细胞（HFF）和FO-1H细胞（已转染人β2-m基因的FO-1细胞，恢复细胞表面的MHC I表达）进行比较。根据HCMV立即早期1（IE-1）抗原表达判断，HCMV能够感染FO-1细胞，尽管效率略低于HFF。然而，HCMV晚期（L）抗原的表达和病毒的产生在FO-1细胞中显著低于HFF。FO-1H转染子的分析显示IE-1和L HCMV抗原的表达与缺乏MHC I的FO-1细胞相当。在接种前用丁酸钠处理FO-1和FO-1H细胞并不改变任一细胞类型中MHC I的表达，但确实增加了两种细胞类型对HCMV感染的易感性，以及L抗原的表达和病毒的产生。这些研究表明HCMV感染FO-1细胞与β2-m和MHC I类分子表达无关。
To study the roles ofβ2-microglobulin (β2-m)and major histocompatibility complex (MHC) class I expression in human cytomegalovirus (HCMV) infection, the ability of HCMV strain AD-169 to infect and replicate in a human melanoma cell line (FO-1), which is β,-m- deficient and cannot express MHC class I on its cell surface, was examined. Susceptibility of FO-1 cells was compared with human foreskin fibroblasts (HFF) and FO-1H cells (FO-1 cells that have been transfected with the humanβ2-mgene, restoring MHC I expression on the cell surface). As judged by the HCMV immediate early 1 (IE-1) antigen expression, HCMV was able to infect FO-1 cells, although somewhat less efficiently than HFF. However, the expression of HCMV late (L) antigen and the production of virus was significantly less for FO-1 cells than for HFF. Analysis of the FO-1H transfectants revealed that expression of IE-1 and L HCMV antigens was comparable to FO-1 cells, which lack MHC I. Treatment of FO-1 and FO-1H cells with sodium butyrate prior to inoculation did not alter the expression of MHC I in either cell type, but did increase susceptibility of both cell types to HCMV infection, as well as the expression of L antigens and production of virus. These studies indicate that HCMV infection of FO-1 cells is independent ofβ2-m and MHC class I expression.
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影响因子：
15.9
作者：
DURSO, CM;WANG, ZG;FERRONE, S
通讯作者：
FERRONE, S
对鼠巨细胞病毒的抵抗力与小鼠的主要组织相容性复合体有关。
影响因子：
3.8
作者：
J. Chalmer;J. Mackenzie;N. Stanley
通讯作者：
N. Stanley
影响因子：
2.9
作者：
SEGE, K;RASK, L;PETERSON, PA
通讯作者：
PETERSON, PA
影响因子：
3.8
作者：
GRUNDY, JE;MCKEATING, JA;GRIFFITHS, PD
通讯作者：
GRIFFITHS, PD
影响因子：
64.5
作者：
BARNSTABLE, CJ;BODMER, WF;ZIEGLER, A
通讯作者：
ZIEGLER, A