Effects of propofol and sevoflurane on aquaporin-4 and aquaporin-9 expression in patients performed gliomas resection
Effects of propofol and sevoflurane on aquaporin-4 and aquaporin-9 expression in patients performed gliomas resection
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丙泊酚和七氟醚对胶质瘤切除患者水通道蛋白4和水通道蛋白9表达的影响
DOI：
10.1016/j.brainres.2015.05.042
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Fei-fei Chang
中科院分区：
文献类型：
--
作者：
Chun-yu Song;Hua Qian;Shu-jun Lu;Fei-fei Chang
脑胶质瘤切除术后脑水肿是患者的一大威胁。一些研究表明，全身麻醉药物如异丙酚具有神经保护作用。水通道蛋白4 （Aquaporin-4, AQP4）和水通道蛋白9 （Aquaporin-9, AQP9）在各种条件下维持脑水稳态中起重要作用。本研究的目的是比较异丙酚或七氟醚对胶质瘤切除术患者AQP4和AQP9表达的影响。本研究纳入30例脑胶质瘤切除术患者。患者随机分为两组：异丙酚组和七氟醚组。获取新鲜人胶质瘤标本，采用苏木精伊红（HE）染色、免疫组织化学染色和Western blot分析，观察AQP4和AQP9的表达情况。免疫组化染色显示，异丙酚组AQP4阳性细胞比例（14.3±4.61%）明显低于七氟醚组（37.3±10.01%）（n=15，P<0.05）。异丙酚组与七氟醚组AQP9阳性细胞百分比比较，差异无统计学意义（25.8±2.67 vs 28.1±7.81%,n=15,P < 0.05）。免疫印迹分析证实了免疫组化结果。异丙酚组AQP4蛋白水平显著低于七氟醚组（1.4±0.13比1.7±0.12，P<0.05）。Western blot分析显示，异丙酚组与七氟醚组AQP9蛋白表达差异无统计学意义（2.0±0.13比2.1±0.13,P < 0.05,n=6）。异丙酚组AQP4表达低于七氟醚组。结果提示异丙酚可抑制AQP4的表达。
Post-operative cerebral edema is a threat for patients performed gliomas resection. Some studies have shown that general anesthesia drugs, such as, propofol had neuroprotective effect. Aquaporin-4 (AQP4) and Aquaporin-9 (AQP9) play an important role in maintaining brain water homeostasis under various conditions. The aim of this study was to compare the effect of propofol or sevoflurane on expression of AQP4 and AQP9 in patients performed gliomas resection. 30 patients performed gliomas resection were included in this study. The patients were randomly divided into two groups: propofol group and sevoflurane group. Fresh human gliomas specimens were obtained and hematoxylin eosin (HE) staining, immunohistochemical staining and Western blot analysis were used for observation of the expression of AQP4 and AQP9. The immunohistochemical staining of the sections showed that the percentage of AQP4 positive cells in the propofol group (14.3±4.61%) was significantly lower than that in sevoflurane group (37.3±10.01%) (n=15,P<0.05). There was no significant difference in the percentage of AQP9 positive cells in propofol group and sevoflurane group (25.8±2.67 versus 28.1±7.81%,n=15,P>0.05). Western blot analysis confirmed the immunohistochemistry results. AQP4 protein level in propofol group was significantly lower than that in sevoflurane group (1.4±0.13 versus 1.7±0.12,P<0.05). Western blot analysis did not show any difference of expression of AQP9 protein between the propofol group and sevoflurane group (2.0±0.13 versus 2.1±0.13,P>0.05,n=6). AQP4 expression was lower in patients of propofol group than that in sevoflurane group. Our results suggested that propofol could inhibit the expression of AQP4.
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影响因子：
4.2
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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