LncRNA regulates tomato fruit cracking by coordinating gene expression via a hormone-redox-cell wall network
LncRNA regulates tomato fruit cracking by coordinating gene expression via a hormone-redox-cell wall network
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LncRNA 通过激素-氧化还原-细胞壁网络协调基因表达来调节番茄果实开裂
DOI：
10.1186/s12870-020-02373-9
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发表时间：
2019-09
影响因子：
5.3
通讯作者：
Jiang Fangling
中科院分区：
文献类型：
--
作者：
Xue Lingzi;Sun Mintao;Wu Zhen;Yu Lu;Yu Qinghui;Tang Yaping;Jiang Fangling
研究背景裂果是果树生产中的主要病害之一，在不适宜的环境条件下极易发生。植物对非生物胁迫的反应包括感知、整合和响应胁迫信号，并通过调控相关基因的表达来实现。裂果也是一种由非生物胁迫引起的生理病害。据报道，一个或几个基因可能调控果实开裂。然而，几乎没有这些报告涉及开裂监管networks.ResultsHere，RNA表达在0小时，8小时和30小时饱和灌溉处理的水果从两个对比的番茄基因型，“LA 1698”（抗开裂，CR）和“LA 2683”（开裂敏感，CS），通过mRNA和lncRNA测序进行了分析。差异表达mRNA的GO途径主要集中在“激素代谢过程”、“细胞壁组织”、“氧化还原酶活性”和“催化活性”类别。基因表达谱分析显示，差异表达显著的基因有Solyc 02 g 080530. 3（过氧化物，POD），Solyc01g008710.3（甘露聚糖内切-1，4-β-甘露糖苷酶，MAN），Solyc08g077910.3（扩展，EXP），Solyc09g075330.3（果胶酯酶，PE），Solyc07g055990.3（木葡聚糖内切转葡糖基酶-水解酶7，XTH 7），Solyc12g011030.2（木葡聚糖内切转葡糖基酶-水解酶9，XTH 9），Solyc10g080210.2（多聚半乳糖醛酸酶-2，PG 2），Solyc08g081010.2（γ-谷氨酰半胱氨酸合成酶，γ-GCS），Solyc09g008720.2（乙烯受体，ER），Solyc11g042560.2（乙烯应答转录因子4，ERF 4）等。（XLOC_16662和XLOC_033910等）调节其邻近基因的表达，和番茄裂果相关基因构建了影响番茄裂果的lncRNA-mRNA网络。具体来说，lncRNA调控植物激素（生长素、乙烯）和ROS（H2 O2）信号转导以及许多与细胞壁相关的mRNA（EXP、PG、XTH），还有一些lncRNA（XLOC_16662和XLOC_033910等）。
BackgroundFruit cracking occurs easily under unsuitable environmental conditions and is one of the main types of damage that occurs in fruit production. It is widely accepted that plants have developed defence mechanisms and regulatory networks that respond to abiotic stress, which involves perceiving, integrating and responding to stress signals by modulating the expression of related genes. Fruit cracking is also a physiological disease caused by abiotic stress. It has been reported that a single or several genes may regulate fruit cracking. However, almost none of these reports have involved cracking regulatory networks.ResultsHere, RNA expression in 0 h, 8 h and 30 h saturated irrigation-treated fruits from two contrasting tomato genotypes, ‘LA1698’ (cracking-resistant, CR) and ‘LA2683’ (cracking-susceptible, CS), was analysed by mRNA and lncRNA sequencing. The GO pathways of the differentially expressed mRNAs were mainly enriched in the ‘hormone metabolic process’, ‘cell wall organization’, ‘oxidoreductase activity’ and ‘catalytic activity’ categories. According to the gene expression analysis, significantly differentially expressed genes included Solyc02g080530.3 (Peroxide, POD), Solyc01g008710.3 (Mannan endo-1,4-beta-mannosidase, MAN), Solyc08g077910.3 (Expanded, EXP), Solyc09g075330.3 (Pectinesterase, PE), Solyc07g055990.3 (Xyloglucan endotransglucosylase-hydrolase 7, XTH7), Solyc12g011030.2 (Xyloglucan endotransglucosylase-hydrolase 9, XTH9), Solyc10g080210.2 (Polygalacturonase-2, PG2), Solyc08g081010.2 (Gamma-glutamylcysteine synthetase, gamma-GCS), Solyc09g008720.2 (Ethylene receptor, ER), Solyc11g042560.2 (Ethylene-responsive transcription factor 4, ERF4) etc. In addition, the lncRNAs (XLOC_16662 and XLOC_033910, etc) regulated the expression of their neighbouring genes, and genes related to tomato cracking were selected to construct a lncRNA-mRNA network influencing tomato cracking.ConclusionsThis study provides insight into the responsive network for water-induced cracking in tomato fruit. Specifically, lncRNAs regulate the hormone-redox-cell wall network, including plant hormone (auxin, ethylene) and ROS (H2O2) signal transduction and many cell wall-related mRNAs (EXP, PG, XTH), as well as some lncRNAs (XLOC_16662 and XLOC_033910, etc.).
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
6.4
作者：
Seiji Yoshida;M. Tamaoki;Motohide Ioki;D. Ogawa;Yuko Sato;M. Aono;A. Kubo;S. Saji;H. Saji;S. Satoh;N. Nakajima
通讯作者：
Seiji Yoshida;M. Tamaoki;Motohide Ioki;D. Ogawa;Yuko Sato;M. Aono;A. Kubo;S. Saji;H. Saji;S. Satoh;N. Nakajima
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
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影响因子：
5.1
作者：
Yang B;Xia ZA;Zhong B;Xiong X;Sheng C;Wang Y;Gong W;Cao Y;Wang Z;Peng W
通讯作者：
Peng W