Effects of dietary toxic cyanobacteria and ammonia exposure on immune function of blunt snout bream (Megalabrama amblycephala)
Effects of dietary toxic cyanobacteria and ammonia exposure on immune function of blunt snout bream (Megalabrama amblycephala)
复制标题
膳食有毒蓝藻和氨暴露对团头鲂免疫功能的影响
DOI：
10.1016/j.fsi.2018.04.023
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发表时间：
2018-07
影响因子：
4.7
通讯作者：
Zhang Xuezhen
中科院分区：
文献类型：
--
作者：
Xia Hu;Son Ting;Wang Li;Jiang Liangsen;Zhou Qiting;Wang Weimin;Liu Liangguo;Yang Pinhong;Zhang Xuezhen
由水体富营养化引起的蓝藻水华已成为一个世界性的问题。在有毒蓝藻水华降解过程中，氨和微囊藻毒素浓度同时升高，对鱼类产生毒性。到目前为止，微囊藻毒素和氨对鱼类免疫毒性的联合影响还没有报道。将团头鲂（Megalabrama amblycephala）暴露于不同浓度的NH3-N（0、0.06、0.12 mg/L）溶液中，并在饲料中添加15%和30%的有毒蓝藻冻干粉，研究团头鲂对饲料中有毒蓝藻和氨暴露的免疫应答。团头鲂各器官中微囊藻毒素含量大小顺序为头肾>肝>肠>性腺>脾>鳃>干肾>脑>肌肉>心脏。在头肾和脾脏中，MC-LR和MC-RR浓度随NH3-N浓度的增加而显著增加。说明氨氮可能促进团头鲂免疫器官中微囊藻毒素的积累。同时，中毒组鱼头肾淋巴细胞周边间隙增宽，细胞核皱缩，线粒体水肿。此外，在头肾巨噬细胞吞噬活性、呼吸爆发活性、白色血细胞总数以及sIgM、mIgD和sIgZ基因转录水平上，饲料中有毒蓝藻与氨暴露之间存在显著的交互作用。我们的数据清楚地表明，饲料中有毒蓝藻与氨暴露相结合，对团头鲂的免疫毒性表现出协同效应。
Cyanobacterial blooms caused by water eutrophication have become a worldwide problem. During the degradation of toxic cyanobacterial blooms, elevated ammonia and microcystins concentrations co-occur and exert toxicity on fish. Up to now, the combined effect of microcystins and ammonia on fish immunotoxicity has not been reported. The present study investigated immune responses of blunt snout bream (Megalabrama amblycephala) to dietary toxic cyanobacteria and ammonia exposure.Megalobrama amblycephalawere exposed to solutions with different concentrations of NH3–N (0, 0.06, 0.12 mg/L) and fed with diets containing 15% and 30% of toxic cyanobacteria lyophilized powder for 30 d. The microcystins concentration in different organs ofMegalobrama amblycephalawas in the following sequence: head kidney > liver > intestine > gonad > spleen > gill > trunk kidney > brain > muscle > heart. In both head kidney and spleen, the MC-LR and MC-RR concentration increased significantly with increasing NH3–N concentration. It indicates that NH3–N maybe promote the accumulation of microcystins in immune organs ofMegalobrama amblycephala. Meanwhile, broadened peripheral interspace of lymphocytes, nucleus shrivel and edematous mitochondria were observed in head kidney lymphocyte of toxic treatment fish. Moreover, there were significant interactions between dietary toxic cyanobacteria and ammonia exposure on head kidney macrophage phagocytosis activity, respiratory burst activities, total number of white blood cells and the transcriptional levels of sIgM, mIgD and sIgZ genes. Our data clearly demonstrated that dietary toxic cyanobacteria combined with ammonia exposure showed a synergistic effect onMegalobrama amblycephalaimmunotoxicity.
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影响因子：
--
作者：
Paerl HW;Fulton RS 3rd;Moisander PH;Dyble J
通讯作者：
Dyble J
影响因子：
4.1
作者：
Li Wang;Chuanyue Chen;Wanjing Liu;H. Xia;J. Li;Xuezhen Zhang
通讯作者：
Li Wang;Chuanyue Chen;Wanjing Liu;H. Xia;J. Li;Xuezhen Zhang
影响因子：
4.5
作者：
Cazenave, J;Bistoni, MDA;Wunderlin, DA
通讯作者：
Wunderlin, DA
影响因子：
6.6
作者：
Jun Xu;Min Zhang;P. Xie
通讯作者：
Jun Xu;Min Zhang;P. Xie
影响因子：
1.3
作者：
Wei Yang;Fuhui Xiang;L. Liang;Zhou Yang
通讯作者：
Wei Yang;Fuhui Xiang;L. Liang;Zhou Yang