Identification of two iron-repressed periplasmic proteins in Haemophilus influenzae
Identification of two iron-repressed periplasmic proteins in Haemophilus influenzae
复制标题
流感嗜血杆菌中两种铁抑制周质蛋白的鉴定
作者：
R. Harkness;P. Chong;M. Klein
研究了流感嗜血杆菌在限铁生长条件下的蛋白表达。5株b型菌株和4株不可分型菌株在生长过程中被剥夺铁后均表达了约40 kDa的新蛋白。在相同的生长条件下，大多数菌株也表达了约31 kDa的蛋白质。在含铁培养基中培养的细胞不表达40和31 kda蛋白。40- kda和31-kDa蛋白在添加过量硝酸铁的缺铁培养基中不表达，因此可以推断它们的表达受到铁的调节。这些铁抑制蛋白定位于质周间隙。测定了两种蛋白的氨基末端序列。40-kDa蛋白的N端序列与淋病奈瑟菌和脑膜炎奈瑟菌的主要铁结合蛋白Fbp的N端序列相似度为81%。31 kda蛋白序列与已知蛋白序列无同源性。由于在菌株中未发现质粒，因此认为这些蛋白是染色体编码的。
Protein expression by Haemophilus influenzae under iron-limiting growth conditions was examined. The five type b strains and four nontypeable strains studied all expressed a new protein of about 40 kDa when deprived of iron during growth. Most strains also expressed a protein of about 31 kDa under the same growth conditions. Both the 40- and 31-kDa proteins were not expressed by cells grown in iron-replete medium. The 40- and 31-kDa proteins were not expressed in iron-deficient medium to which an excess of ferric nitrate had been added, and therefore it was concluded that their expression was iron regulated. These iron-repressed proteins were localized to the periplasmic space. The amino-terminal sequences of both proteins were determined. The N-terminal sequence of the 40-kDa protein had 81% similarity to the N terminus of Fbp, the major iron-binding protein of Neisseria gonorrhoeae and N. meningitidis. The 31-kDa protein sequence showed no homology with any known protein sequence. As no plasmids were found in the strains, it was concluded that these proteins were chromosomally encoded.
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b 型流感嗜血杆菌分离株外膜蛋白 P2 的纯化和比较。
DOI：
10.1172/jci111017
发表时间：
1983
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
MunsonJr,RS;Shenep,JL;Barenkamp,SJ;Granoff,DM
通讯作者：
Granoff,DM
DOI：
--
发表时间：
1990
期刊：
--
影响因子：
--
作者：
Neilands Jb
通讯作者：
Neilands Jb
血红蛋白结合位点及其与触珠蛋白丝氨酸蛋白酶样活性位点的关系。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Arcoleo,JP;Greer,J
通讯作者：
Greer,J
从巴基斯坦患有下呼吸道感染的儿童中分离出的流感嗜血杆菌的克隆分析。
DOI：
10.1093/infdis/160.4.634
发表时间：
1989
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Weinberg,GA;Ghafoor,A;Ishaq,Z;Nomani,NK;Kabeer,M;Anwar,F;Burney,MI;Qureshi,AW;Musser,JM;Selander,RK
通讯作者：
Selander,RK