The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models.
The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models.
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DOI：
10.1371/journal.pone.0010289
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发表时间：
2010-04-21
期刊：
影响因子：
3.7
通讯作者：
Bishai WR
中科院分区：
文献类型：
--
作者：
Converse PJ;Eisenach KD;Theus SA;Nuermberger EL;Tyagi S;Ly LH;Geiman DE;Guo H;Nolan ST;Akar NC;Klinkenberg LG;Gupta R;Lun S;Karakousis PC;Lamichhane G;McMurray DN;Grosset JH;Bishai WR
有人推测，实验室传代的结核分枝杆菌和重组结核分枝杆菌突变体的毒力可能会由于体外多次传代而降低，而在小鼠或豚鼠气溶胶模型中进行定量毒力测试之前，通过小鼠传代这些菌株可能会增强毒力。通过在小鼠或豚鼠气溶胶或静脉感染模型中用定量器官CFU计数方法检测三个结核分枝杆菌H37Rv样本、一个缺失突变体和一个最近存活的临床分离株，我们可以在两个动物模型中观察到在定量毒力测试之前，结核分枝杆菌菌株在小鼠体内传代后细菌适应性没有增加。通过中性红染色和PDIM分析薄层层析评估的表面脂质表达也未能确定毒力相关性。这些结果表明，在小鼠或豚鼠模型上进行定量毒力测试之前对结核分枝杆菌菌株进行动物传代，并不能增强或恢复可能因体外传代而失去毒力的菌株的效力。在进行基因操作和比较之前，验证亲本菌株的毒力是至关重要的。
It has been hypothesized that the virulence of lab-passaged Mycobacterium tuberculosis and recombinant M. tuberculosis mutants might be reduced due to multiple in vitro passages, and that virulence might be augmented by passage of these strains through mice before quantitative virulence testing in the mouse or guinea pig aerosol models. By testing three M. tuberculosis H37Rv samples, one deletion mutant, and one recent clinical isolate for survival by the quantitative organ CFU counting method in mouse or guinea pig aerosol or intravenous infection models, we could discern no increase in bacterial fitness as a result of passaging of M. tuberculosis strains in mice prior to quantitative virulence testing in two animal models. Surface lipid expression as assessed by neutral red staining and thin-layer chromatography for PDIM analysis also failed to identify virulence correlates. These results indicate that animal passaging of M. tuberculosis strains prior to quantitative virulence testing in mouse or guinea pig models does not enhance or restore potency to strains that may have lost virulence due to in vitro passaging. It is critical to verify virulence of parental strains before genetic manipulations are undertaken and comparisons are made.
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关于结节杆菌的毒力的研究；生物体的生理状态与其致病性的关系。
影响因子：
15.3
作者：
BLOCH, H
通讯作者：
BLOCH, H
DOI：
10.1073/pnas.150227197
发表时间：
2000-07-18
影响因子：
11.1
作者：
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3.2
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作者：
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通讯作者：
Luquin, M
影响因子：
3.6
作者：
Camacho, LR;Ensergueix, D;Guilhot, C
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Guilhot, C