The effect of phenyl tert-butyl nitrone (PBN) on CCl4-induced rat liver injury detected by proton magnetic resonance imaging (MRI) in vivo and electron microscopy (EM).
The effect of phenyl tert-butyl nitrone (PBN) on CCl4-induced rat liver injury detected by proton magnetic resonance imaging (MRI) in vivo and electron microscopy (EM).
复制标题
通过体内质子磁共振成像（MRI）和电子显微镜（EM）检测苯基叔丁基硝酮（PBN）对CCl4诱导的大鼠肝损伤的影响。
DOI：
10.3109/10715769009145691
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
S. Yamashiro
中科院分区：
文献类型：
--
作者：
E. Janzen;R. Towner;S. Yamashiro
急性中毒四氯化碳诱导大鼠肝脏的形态学变化，这是很容易检测的1H-NMR成像技术在原位。CCl 4给药后2 - 4小时，在肝脏小叶中心区观察到高质子信号强度区域。高信号强度的区域归因于CCl 4诱导的损伤导致的局部水肿的形成。CCl 4处理的肝切片的高强度区域的电子显微镜显示特征性的亚细胞变化，其中包括核糖体从粗面内质网（RER）的消失，滑面内质网（SER）的破碎外观，在池中形成空泡和线粒体肿胀。用α-苯基-叔丁基硝酮（PBN），一种自由基自旋捕集剂，在卤烃暴露之前对大鼠进行预处理，发现可减少卤烃引起的肝脏水肿。PBN预处理的四氯化碳暴露大鼠肝切片的电子显微镜检查显示，与对照组相比，亚细胞组织没有明显变化。
Acute intoxication by CCl4 induces morphological changes in rat liver which are readily detectable by 1H-NMR imaging techniques in situ. Two to four hours after the administration of CCl4, regions of high proton signal intensity are observed in the centrilobular region of the liver. The regions of high signal intensity are attributed to the formation of local edema as a result of CCl4-induced damage. Electron microscopy of the high intensity regions of CCl4 treated liver sections revealed characteristic subcellular changes which include the disapperance of ribosomes from the rough endoplasmic reticulum (RER), the fragmented appearance of the smooth endoplasmic reticulum (SER), formation of vacuoles in the cisternae and swelling of the mitochondria. Pretreatment of rats with alpha-phenyl-tert-butyl nitrone (PBN), a free radical spin trap, prior to halocarbon exposure, was found to reduce the halocarbon-induced edema in the liver. Electron microscopy of the PBN pretreated CCl4 exposed rat liver sections revealed no observable changes in subcellular organization when compared to controls.
DOI：
10.1016/s0021-9258(17)38535-6
发表时间：
1986-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Connor;R. Thurman;M. Galizi;R. Mason
通讯作者：
H. Connor;R. Thurman;M. Galizi;R. Mason
影响因子：
10.4
作者：
Janzen EG;Stronks HJ;Dubose CM;Poyer JL;McCay PB
通讯作者：
McCay PB