CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma.
CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma.
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CBFA2T2 与肾细胞癌中的癌症干细胞状态相关
DOI：
10.1186/s12935-017-0473-z
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发表时间：
2017
影响因子：
5.8
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Chen DC;Liang YD;Peng L;Wang YZ;Ai CZ;Zhu XX;Yan YW;Saeed Y;Yu B;Huang J;Gao Y;Liu J;Jiang YZ;Liu M;Chen D
肾细胞癌（RCC）是最常见的肾癌，约占所有原发性肾癌的80-90%。晚期肾细胞癌患者的治疗仍然不令人满意。罕见的癌症干细胞（CSC）被认为是导致当前治疗失败的原因。OncoLnc被用作交互式探索生存相关性的工具。利用siRNA、RT-PCR和Western blotting进行基因操作和表达分析。伤口愈合和侵袭测定用于表型表征。使用Aldefluor测定和FACS分选球培养来确定CSC的“干性”。免疫共沉淀（Co-IP）用于检查OCT 4和CBFA 2 T2之间的相互作用。采用t检验和卡方检验分析统计学意义。CBFA 2 T2表达可显著预测肾癌患者的生存期。CBFA 2 T2基因的敲低可抑制肾癌细胞的体外迁移和侵袭，减少ALDH高表达的肿瘤干细胞的数量。CBFA 2 T2表达对于RCC细胞系中的球体形成能力和癌症干细胞标志物表达是必需的。我们的数据表明，CBFA 2 T2表达与RCC的侵袭性特征相关，并且CBFA 2 T2是通过调节干细胞因子来维持“干性”所必需的，从而突出了CBFA 2 T2作为RCC治疗的潜在治疗靶点。本文的在线版本（10.1186/s12935-017-0473-z）包含补充材料，可供授权用户使用。
Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80–90% of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current treatment. OncoLnc was used as a tool for interactively exploring survival correlations. Gene manipulation and expression analysis were carried out using siRNA, RT-PCR and Western blotting. Wound healing and invasion assays were used for phenotypical characterization. Aldefluor assay and FACS sorting Sphere culture were used to determine the “stemness” of CSCs. Co-Immunoprecipitation (Co-IP) was used to examine the interaction between OCT4 and CBFA2T2. Student’s t-test and Chi square test was used to analyze statistical significance. CBFA2T2 expression can significantly predict the survival of RCC patients. Knocking-down of CBFA2T2 can inhibit cell migration and invasion in RCC cells in vitro, and reduce ALDHhigh CSCs populations. CBFA2T2 expression is necessary for sphere-forming ability and cancer stem cells marker expression in RCC cell lines. Our data suggest that CBFA2T2 expression correlates with aggressive characteristics of RCC and CBFA2T2 is required for maintenance of “stemness” through regulation of stem cells factors, thereby highlighting CBFA2T2 as a potential therapeutic target for RCC treatment. The online version of this article (10.1186/s12935-017-0473-z) contains supplementary material, which is available to authorized users.
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影响因子：
--
作者：
Cheng B;Yang G;Jiang R;Cheng Y;Yang H;Pei L;Qiu X
通讯作者：
Qiu X
影响因子：
16.6
作者：
Li J;Yu B;Deng P;Cheng Y;Yu Y;Kevork K;Ramadoss S;Ding X;Li X;Wang CY
通讯作者：
Wang CY
影响因子：
3.8
作者：
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通讯作者：
Anaya, Jordan
影响因子：
4.8
作者：
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通讯作者：
Camussi, Giovanni
影响因子：
5.2
作者：
Gassenmaier, Maximilian;Chen, Dong;Pohla, Heike
通讯作者：
Pohla, Heike