Mutant prohead RNAs in the in vitro packaging of bacteriophage phi 29 DNA-gp3.
Mutant prohead RNAs in the in vitro packaging of bacteriophage phi 29 DNA-gp3.
复制标题
噬菌体 phi 29 DNA-gp3 体外包装中的突变前头 RNA。
DOI：
10.1016/0022-2836(92)90257-k
复制
发表时间：
1992
影响因子：
5.6
通讯作者：
Anderson,D
中科院分区：
文献类型：
--
作者：
Wichitwechkarn,J;Johnson,D;Anderson,D
The 174-base prohead RNA encoded by bacteriophage φ29 ofBacillus subtilis, essential for packaging of the DNA-gp3 (DNA-gene product 3) complex, was expressed efficiently from the cloned gene. Computer programs for RNA structure analysis were used to fold hypothetical RNA mutants and thus to target mutagenesis of the RNA for studies of structure and function. Five mutants of the RNA were then produced by oligonucleotide-directed mutagenesis that were altered in the primary sequence at selected sites; two of these mutants were predicted to be altered in secondary structure from a model established previously by a phylogenetic analysis. The binding of the32P end-labeled mutant RNAs to RNA-free proheads was comparable with that of the wild-type RNA. However, the capability of the mutant RNAs to reconstitute RNA-free proheads for DNA-gp3 packaging in the definedin vitrosystem and for assembly of phage in RNA-free extracts was variable, depending upon the alteration. Changes of highly conserved bases that retained the predicted secondary structure of the RNA model were tolerated to a much greater extent than changes predicted to alter the RNA secondary structure.
登录
查看更多内容
影响因子：
56.9
作者：
GUO, PX;ERICKSON, S;ANDERSON, D
通讯作者：
ANDERSON, D
噬菌体 φ29 DNA 包装 ATP 酶的 RNA 依赖性
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
S. Grimes;D. Anderson
通讯作者：
D. Anderson
DOI：
10.1101/sqb.1987.052.01.017
发表时间：
1987-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
TURNER, DH;SUGIMOTO, N;KIERZEK, R
通讯作者：
KIERZEK, R
自剪接 II 族内含子中的结构-功能关系：线粒体内含子 aI5 的结构域 II 的大部分对于自剪接不是必需的。
影响因子：
14.9
作者：
J. Kwakman;Danielle Konings;H. Pel;L. Grivell
通讯作者：
L. Grivell
DOI：
10.1073/pnas.83.24.9373
发表时间：
1986-12-01
影响因子：
11.1
作者：
FREIER, SM;KIERZEK, R;TURNER, DH
通讯作者：
TURNER, DH