Quantitative dynamics of VE-cadherin at endothelial cell junctions at a glance: basic requirements and current concepts
Quantitative dynamics of VE-cadherin at endothelial cell junctions at a glance: basic requirements and current concepts
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内皮细胞连接处 VE-钙粘蛋白的定量动力学概览：基本要求和当前概念
DOI：
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
H. Schnittler
中科院分区：
文献类型：
--
作者：
J. Seebach;Jiahui Cao;H. Schnittler
血管内皮的细胞间连接是显示高度可塑性的动态结构，这是有助于它们调节许多生理和病理过程所需的，包括单层完整性、屏障功能、伤口愈合和血管生成。血管内皮钙粘蛋白（VE-cadherin）通过连接蛋白连接到肌动蛋白细胞骨架，两者都是内皮连接调节的关键结构，因此是许多研究的焦点。基于荧光的活细胞成像是研究活细胞动态重塑的首选方法。虽然这些方法已成功地应用于许多细胞类型，但内皮连接动力学的研究在很长一段时间内受到限制，因为它们在很大程度上抵抗使用许多经典方案的转染。应用基于病毒的基因转导技术，再加上先进的显微镜，现在允许充分表达的荧光标记的连接定位的蛋白质在内皮细胞和延时记录在很长一段时间。使用高度时空分辨的荧光显微镜，结果表明，内皮连接显示广泛的连接异质性在亚细胞水平上，这在很大程度上限制了可用软件的自动定量的事实。最近的工作描述了开放的软件工具，以定量分析大量的荧光为基础的图像数据，无论是单一的或融合的上皮和内皮细胞。基于定量VE-钙粘蛋白和肌动蛋白动力学的新的关键球员，机制和概念已被提出，控制内皮连接动力学。在这里，我们的目的是总结该领域的最新发展。
Intercellular junctions of the vascular endothelium are dynamic structures that display a high degree of plasticity, which is required to contribute to their regulation of many physiological and pathological processes including monolayer integrity, barrier function, wound healing and angiogenesis. Vascular endothelial cadherin (VE-cadherin) is connected via catenins to the actin cytoskeleton, both of which are key structures in endothelial junction regulation, and thus are the focus of much investigation. Fluorescence-based live cell imaging is the method of choice to study dynamic remodeling in living cells. Although these methods have been successfully applied to many cell types, investigations of endothelial junction dynamics were for a long time limited as they are largely resistant to transfection using many classical protocols. Application of virus-based gene transduction techniques, together with advanced microscopy, now allows both sufficient expression of fluorescence tagged junction-localized proteins in the endothelium and time-lapse recording over long periods. Using highly spatiotemporally resolved fluorescence microscopy it turned out that endothelial junctions display extensive junction heterogeneity at the subcellular level; a fact that largely limits automated quantification by available software. Recent work describes open software tools to quantitatively analyze large amounts of fluorescence-based image data in either single or confluent epithelial and endothelial cells. Based on quantitative VE-cadherin and actin dynamics novel key players, mechanisms and concepts have been suggested that control endothelial junction dynamics. Here we aim to summarize the recent developments in the field.
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影响因子：
6.7
作者：
J. Seebach;H. Mädler;B. Wojciak-Stothard;H. Schnittler
通讯作者：
J. Seebach;H. Mädler;B. Wojciak-Stothard;H. Schnittler
影响因子：
7.5
作者：
Harris ES;Nelson WJ
通讯作者：
Nelson WJ
影响因子：
6.4
作者：
Aleksandrowicz, Paulina;Marzi, Andrea;Schnittler, Hans-Joachim
通讯作者：
Schnittler, Hans-Joachim
CellBorderTracker，一种在亚细胞水平定量分析时空内皮连接动态的新工具
影响因子：
2.3
作者：
Seebach;Lindemann;Brinkmann;Bogdan;Schnittler
通讯作者：
Schnittler
影响因子：
62.1
作者：
Newman, Robert H.;Fosbrink, Matthew D.;Zhang, Jin
通讯作者：
Zhang, Jin