A dynamic microbial community with high functional redundancy inhabits the cold, oxic subseafloor aquifer.
A dynamic microbial community with high functional redundancy inhabits the cold, oxic subseafloor aquifer.
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DOI：
10.1038/ismej.2017.187
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Huber JA
中科院分区：
文献类型：
--
作者：
Tully BJ;Wheat CG;Glazer BT;Huber JA
以岩石为宿主的海底下地壳含水层是微生物生命的储存库，可能影响全球海洋生物地球化学循环。在这里，我们利用宏基因组库的地壳流体样本从北池，位于大西洋中脊，一个网站与冷，氧化海底流体循环内的上基底查询微生物多样性的侧翼。在2年的时间里收集了21个样品，以研究潜在的微生物代谢和群落动态。我们观察到的地球化学特征在2年的微小变化，但在地壳流体中存在的微生物群落经历了大的变化，在占主导地位的分类群体。195宏基因组组装的基因组（MAG）的分析产生的数据集，并揭示了石和自养过程之间的连接，连接碳固定的硫化物，硫，硫代硫酸盐，氢，和亚铁的变形菌门的成员，特别是阿尔法，伽马和Zetaproteobacteria，Epsilonbacteraeota和浮游菌的氧化。尽管有氧条件下，分析的MAG表明，微生物群落的成员准备利用缺氧或缺氧条件，通过使用微好氧细胞色素，如cbb 3-和BD型细胞色素，和替代电子受体，如硝酸盐和硫酸盐。从MAG的时间和空间趋势显示了高度的功能冗余，不相关的转变微生物群落的成员资格，这表明在介导海底下生物地球化学循环的功能稳定性。总的来说，在多个地点的重复采样，以及数百个基因组的成功分箱，为调查寒冷、含氧的地壳含水层中的微生物群落提供了前所未有的数据集。
The rock-hosted subseafloor crustal aquifer harbors a reservoir of microbial life that may influence global marine biogeochemical cycles. Here we utilized metagenomic libraries of crustal fluid samples from North Pond, located on the flanks of the Mid-Atlantic Ridge, a site with cold, oxic subseafloor fluid circulation within the upper basement to query microbial diversity. Twenty-one samples were collected during a 2-year period to examine potential microbial metabolism and community dynamics. We observed minor changes in the geochemical signatures over the 2 years, yet the microbial community present in the crustal fluids underwent large shifts in the dominant taxonomic groups. An analysis of 195 metagenome-assembled genomes (MAGs) were generated from the data set and revealed a connection between litho- and autotrophic processes, linking carbon fixation to the oxidation of sulfide, sulfur, thiosulfate, hydrogen, and ferrous iron in members of the Proteobacteria, specifically the Alpha-, Gamma- and Zetaproteobacteria, the Epsilonbacteraeota and the Planctomycetes. Despite oxic conditions, analysis of the MAGs indicated that members of the microbial community were poised to exploit hypoxic or anoxic conditions through the use of microaerobic cytochromes, such as cbb3- and bd-type cytochromes, and alternative electron acceptors, like nitrate and sulfate. Temporal and spatial trends from the MAGs revealed a high degree of functional redundancy that did not correlate with the shifting microbial community membership, suggesting functional stability in mediating subseafloor biogeochemical cycles. Collectively, the repeated sampling at multiple sites, together with the successful binning of hundreds of genomes, provides an unprecedented data set for investigation of microbial communities in the cold, oxic crustal aquifer.
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影响因子：
4.4
作者：
Barco, Roman A.;Emerson, David;Edwards, Katrina J.
通讯作者：
Edwards, Katrina J.
影响因子：
4.4
作者：
Huber, JA;Butterfield, DA;Baross, JA
通讯作者：
Baross, JA
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
2.8
作者：
Fisher, Andrew T.;Wheat, C. Geoffrey
通讯作者：
Wheat, C. Geoffrey
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A