Genome-wide association of yield traits in a nested association mapping population of barley reveals new gene diversity for future breeding.
Genome-wide association of yield traits in a nested association mapping population of barley reveals new gene diversity for future breeding.
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DOI：
10.1093/jxb/ery178
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发表时间：
2018-07-18
影响因子：
6.9
通讯作者：
Flavell AJ
中科院分区：
文献类型：
--
作者：
Sharma R;Draicchio F;Bull H;Herzig P;Maurer A;Pillen K;Thomas WTB;Flavell AJ
从25个不同的野生大麦品系中发现产量相关的谷物性状的QTL热点揭示了改良栽培大麦的新机会。为了探索野生大麦作为育种中有用的等位基因的来源，我们使用嵌套关联作图群体HEB-25进行了全基因组关联扫描，HEB-25包含25个不同的外来大麦基因组，叠加在约70%的栽培大麦遗传背景上。在德国和苏格兰对1420个HEB-25株系的9个产量相关籽粒性状进行了为期2年的试验，并进行了不同氮肥施用量的试验。表型数据与5398个基于基因的单核苷酸多态性（SNP）标记的基因型得分相关。共鉴定了96个数量性状位点（QTL）区域，其中大部分与已知的控制大麦开花时间（Ppd-H2、HvCEN、HvGI、VRN-H1和VRN-H3）和穗形态（VRS 3、VRS 1、VRS 4和INT-C）的主效基因共定位。14个QTL热点，至少有三个性状相吻合，也被确定，其中几个共定位与大麦直系同源基因控制水稻籽粒尺寸。大多数等位基因效应是特定于地理位置和/或外来亲本基因型。本研究表明外来大麦种质中存在与产量相关性状的有益等位基因，并为育种家未来改进这些性状提供了候选等位基因。
Discovering QTL hotspots for yield-related grain traits in a population developed from 25 diverse wild barley lines reveals new opportunities for improving cultivated barley. To explore wild barley as a source of useful alleles for yield improvement in breeding, we have carried out a genome-wide association scan using the nested association mapping population HEB-25, which contains 25 diverse exotic barley genomes superimposed on an ~70% genetic background of cultivated barley. A total of 1420 HEB-25 lines were trialled for nine yield-related grain traits for 2 years in Germany and Scotland, with varying N fertilizer application. The phenotypic data were related to genotype scores for 5398 gene-based single nucleotide polymorphism (SNP) markers. A total of 96 quantitative trait locus (QTL) regions were identified across all measured traits, the majority of which co-localize with known major genes controlling flowering time (Ppd-H2, HvCEN, HvGI, VRN-H1, and VRN-H3) and spike morphology (VRS3, VRS1, VRS4, and INT-C) in barley. Fourteen QTL hotspots, with at least three traits coinciding, were also identified, several of which co-localize with barley orthologues of genes controlling grain dimensions in rice. Most of the allele effects are specific to geographical location and/or exotic parental genotype. This study shows the existence of beneficial alleles for yield-related traits in exotic barley germplasm and provides candidate alleles for future improvement of these traits by the breeder.
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影响因子：
5.4
作者：
Dunford, RP;Griffiths, S;Laurie, DA
通讯作者：
Laurie, DA
DOI：
10.1300/j144v05n01_06
发表时间：
2002-01-01
期刊：
Journal of Crop Production
影响因子：
--
作者：
Igartua, E.;Hayes, P. M.;Mather, D. E.
通讯作者：
Mather, D. E.
影响因子：
2.3
作者：
Mather, DE;Tinker, NA;Kasha, KJ
通讯作者：
Kasha, KJ
影响因子：
3.3
作者：
Faure, Sebastien;Higgins, Janet;Laurie, David A.
通讯作者：
Laurie, David A.
影响因子：
56.9
作者：
Buckler, Edward S.;Holland, James B.;McMullen, Michael D.
通讯作者：
McMullen, Michael D.