Measurement of Hypothalamic Glucose Under Euglycemia and Hyperglycemia by MRI at 3T.
Measurement of Hypothalamic Glucose Under Euglycemia and Hyperglycemia by MRI at 3T.
复制标题
MRI在3T下测量下丘脑葡萄糖和高血糖下的下丘脑葡萄糖。
DOI：
10.1002/jmri.25383
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Öz G
中科院分区：
文献类型：
--
作者：
Joers JM;Deelchand DK;Kumar A;Moheet A;Seaquist E;Henry PG;Öz G
评估使用临床磁共振（MR）系统和MR波谱（MRS）测量人体下丘脑（全身葡萄糖调节的中心结构）葡萄糖浓度变化的可行性。在基线（正常）和11分钟阻滞中约65-70分钟长的高血糖钳夹期间，在3 T下获得6名健康受试者的MR频谱（半激光，TE = 28 msec）时间序列，定位于双侧下丘脑（约1.6 ml），并使用交错T1 FLASH图像回顾性评估头部运动，并跟踪脑脊液（CSF）部分体积的变化。LC模型用于定量葡萄糖和牛磺酸浓度之和[Glc+Tau]，沿着其相关Cramér-Rao下限（CRLB）。光谱质量允许在高血糖症期间的35/36个时间点中以CRLB <25%定量[Glc+Tau]（由于这些代谢物之间的高度负相关性而报告的总和）。在所有受试者中观察到高血糖症患者[Glc+Tau]增加，但在血糖目标≥300 mg/dl的受试者中最可靠。对于这些受试者，[Glc+Tau]基线（n = 4）为1.5（±0.3，SD）mM，在钳夹开始后≥25分钟采集的时间点增加至4.5（±1.1）mM（n = 16），15/16个时间点的基线和高血糖之间的95%置信区间（CI）无重叠。初步分析显示下丘脑-血糖浓度之间呈线性（1：5）关系。通过使用回顾性运动跟踪、CSF校正、预定质量验收标准和高血糖血糖水平≥300 mg/dl，使用标准3 T扫描仪和短回波半激光序列测量人下丘脑中的葡萄糖浓度变化是可行的。2
To evaluate the feasibility of using a clinical magnetic resonance (MR) system and MR spectroscopy (MRS) to measure glucose concentration changes in the human hypothalamus, a structure central to whole-body glucose regulation. A time series of MR spectra (semi-LASER, TE = 28 msec), localized to the bilateral hypothalamus ~1.6 ml) were obtained at 3T in six healthy subjects at baseline (euglycemia) and during a ~65–70-minute-long hyperglycemic clamp in 11-minute blocks with interleaved T1 FLASH images to retrospectively assess head motion, and track changes in cerebrospinal fluid (CSF) partial volume. The LCModel was used to quantify the sum of glucose and taurine concentrations, [Glc+Tau], along with their associated Cramér-Rao lower bounds (CRLB). Spectral quality allowed quantification of [Glc+Tau] (sum reported due to high negative correlation between these metabolites) with CRLB <25% in 35/36 timepoints during hyperglycemia. Increased [Glc+Tau] was observed with hyperglycemia in all subjects, but most reliably in those with plasma glucose targets ≥300 mg/dl. For these subjects, [Glc+Tau]baseline (n = 4) was 1.5 (±0.3, SD) mM, and increased to 4.5 (±1.1) mM (n = 16) for timepoints acquired ≥25 minutes after onset of the clamp, with 15/16 timepoints having no overlap of 95% confidence intervals (CIs) between baseline and hyperglycemia. Preliminary analysis revealed a linear (1:5) relationship between hypothalamus–blood glucose concentrations. It is feasible to measure glucose concentration changes in the human hypothalamus using a standard 3T scanner and a short-echo semi-LASER sequence by utilizing retrospective motion tracking, CSF correction, predetermined quality acceptance criteria, and hyperglycemic blood glucose levels ≥300 mg/dl. 2
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DOI：
10.1007/s00018-015-2079-8
发表时间：
2016-02
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Rooijackers HM;Wiegers EC;Tack CJ;van der Graaf M;de Galan BE
通讯作者：
de Galan BE
DOI：
10.1097/00004647-199605000-00009
发表时间：
1996-05-01
影响因子：
6.3
作者：
Gruetter, R;Novotny, EJ;Shulman, RG
通讯作者：
Shulman, RG
影响因子：
64.8
作者：
Liu, YJ;Gao, JH;Fox, PT
通讯作者：
Fox, PT
影响因子：
2.5
作者：
Mangia, S;Tkác, I;Ugurbil, K
通讯作者：
Ugurbil, K
影响因子：
3.3
作者：
Deelchand, Dinesh K.;Adanyeguh, Isaac M.;Emir, Uzay E.;Nguyen, Tra-My;Valabregue, Romain;Henry, Pierre-Gilles;Mochel, Fanny;Oez, Guelin
通讯作者：
Oez, Guelin