Light-Dark Open Field (LDOF): A novel task for sensitive assessment of anxiety.
Light-Dark Open Field (LDOF): A novel task for sensitive assessment of anxiety.
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DOI：
10.1016/j.jneumeth.2021.109325
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发表时间：
2021-11-01
影响因子：
3
通讯作者：
Vazdarjanova AI
中科院分区：
文献类型：
--
作者：
Shanazz K;Dixon-Melvin R;Bunting KM;Nalloor R;Vazdarjanova AI
精神疾病的临床前研究通常包括焦虑样行为的测量。有几个任务可以达到这个目的，但是因为焦虑是复杂的，有无数的焦虑刺激，研究人员经常被迫使用多个任务。光暗开放领域（LDOF）将来自两个这样的任务（光暗盒和开放领域）的概念结合到一个任务中，具有增强对焦虑样行为的辨别的协同效应。我们的目标是通过增加阴影来增加旷场任务的灵敏度，在概念上类似于亮-暗盒，即使在强光下也能检测到隐藏的差异，这是高度致焦虑的。由此产生的LDOF允许同时评估由于强光和开放空间引起的焦虑，同时保留了使用自定义指数评估每个影响的能力。此外，它保留了开放领域的所有优势和措施。使用自定义创建的指数，从措施中收集的LDOF可以评估焦虑引起的光，开放空间，或光和开放空间相结合，从而阐明焦虑诱导因素。使用两种品系的大鼠：远交品系Sprague-Dawley（SD）和表现出高特质焦虑的品系刘易斯大鼠，我们发现，用明-暗开放视野可以实现对焦虑样行为的增加的辨别。LDOF允许研究人员提取开放领域的传统测量，包括有关运动和习惯化的有价值的信息，同时增加了一层具有明暗成分的区分。由于LDOF是两个不同测试的组合，因此与连续运行多个实验相比，它节省了时间，然后需要进行平衡以减少伪影和任务顺序效应。此外，即使传统的焦虑任务已经达到其灵敏度上限（即在明亮的光线条件下），它也能检测到差异。我们提出了光暗开放领域：一个简单的修改现有的开放领域的设备，结合方面的光暗盒与阴影的增加。阴影（黑暗周长）允许增加在检测焦虑样行为的歧视。比较刘易斯和SD大鼠的焦虑样行为，使我们能够发展的结构和表面效度的LDOF，以及证明其敏感性，即使在明亮的光线条件下。此外，我们开发了3个指数，允许人们从一组数据中解析出两种焦虑刺激的效果-明亮的光线和开放的空间。
Pre-clinical studies of psychiatric disorders often include a measure of anxiety-like behavior. Several tasks exist that serve this purpose, but because anxiety is complex with a myriad of anxiogenie stimuli, researchers are often compelled to use multiple tasks. The Light-Dark Open Field (LDOF) combines concepts from two such tasks, Light-Dark Box and Open Field, into one task with the synergistic effect of enhanced discrimination of anxiety-like behavior. Our goal was to increase the sensitivity of the Open Field task with the addition of a shadow, conceptually similar to the Light-Dark Box, to detect concealed differences even under bright light, which is highly anxiogenie. The resulting LDOF allows assessment of anxiety due to bright light and open space simultaneously, while retaining the ability to assess the impact of each with custom indices. In addition, it maintains all the advantages and measures of the Open Field. Using custom created indices from measures collected in the LDOF one can assess anxiety induced by light, open space, or light and open space combined and thus elucidate anxiety-inducing factors. Using two strains of rats: an outbred strain, Sprague-Dawley (SD), and a strain that exhibits high trait anxiety, Lewis rats, we found that increased discrimination for anxiety-like behavior can be achieved with the Light-Dark Open Field. The LDOF allows researchers to extract the traditional measures of an Open Field, including valuable information about locomotion and habituation while adding a further layer of discrimination with the light-dark component. Because the LDOF is a combination of two different tests, it saves time compared to running multiple experiments in series that then need to be counterbalanced to reduce artefacts and task order effects. In addition, it detects differences even when traditional tasks of anxiety have reached their ceiling sensitivity (i.e. EPM under bright light conditions). We present the Light-Dark Open Field: a simple modification of an existing Open Field apparatus that incorporates aspects of the Light-Dark Box with the addition of a shadow. The shadow (Dark Perimeter) allows for increased discrimination in detecting anxiety-like behaviors. Comparison of anxiety-like behavior between Lewis and SD rats allowed us to develop the construct and face validity of the LDOF as well as demonstrate its sensitivity even under bright light conditions. In addition, we developed 3 indices that allow one to parse out, from one set of data, the effect of two anxiogenie stimuli- bright light and open space.
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DOI：
10.1196/annals.1390.006
发表时间：
2007-01-01
期刊：
REWARD AND DECISION MAKING IN CORTICOBASAL GANGLIA NETWORKS
影响因子：
--
作者：
Balleine, Bernard W.;Ostlund, Sean B.
通讯作者：
Ostlund, Sean B.
影响因子：
2.7
作者：
IVINSKIS, A
通讯作者：
IVINSKIS, A
影响因子：
2.9
作者：
Pati, Sthitapranjya;Sood, Ankit;Vaidya, Vidita A.
通讯作者：
Vaidya, Vidita A.
影响因子：
3.6
作者：
BRITTON, DR;BRITTON, KT
通讯作者：
BRITTON, KT
影响因子：
2.9
作者：
BEUZEN, A;BELZUNG, C
通讯作者：
BELZUNG, C