Effect of azaline B on follicular development and functions in the hamster.
Effect of azaline B on follicular development and functions in the hamster.
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DOI：
10.1016/j.mce.2014.11.011
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发表时间：
2015-01-15
影响因子：
4.1
通讯作者：
Roy, Shyamal K.
中科院分区：
文献类型：
--
作者：
Chakraborty, Prabuddha;Roy, Shyamal K.
The usefulness of azaline B, a GnRH antagonist, in suppressing gonadotropin secretion in the golden hamster was examined by examining follicular development, steroidogenesis and expression of steroidogenic enzymes. Serum levels of P and E declined significantly, while FSH or LH was undetectable in azaline B-treated hamsters. FSH significantly increased serum E levels, whereas LH upregulated serum P levels. The formation of antral follicles ceased in azaline-treated hamsters, but was reversed by FSH with or without LH supplement. FSH also activated the primordial follicle pool resulting in increased formation of primary and preantral follicles. Further, an increasing trend in the formation of preantral follicles in response to E or E + P, and the formation of antral follicles in response to E + P treatment was evident. The level of Cyp11a1 mRNA increased markedly in LH− or LH + FSH-treated hamsters, whereas FSH with or without LH upregulated Cyp17a1, Cyp19a1 and Fshr mRNA expression. E without or with P also upregulated ovarian Cyp19a1 mRNA expression. The expression of enzyme protein corroborated the mRNA data. In summary, azaline B is an efficient GnRH antagonist in the hamster, and will be useful in studying the selective effect of gonadotropins on ovarian functions without disrupting the physiological functions of other hormones in ovarian cells.
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影响因子：
6.7
作者：
Hoang, Yvonne D.;McTavish, Kirsten J.;Chang, R. Jeffrey;Shimasaki, Shunichi
通讯作者：
Shimasaki, Shunichi
DOI：
10.1073/pnas.95.23.13612
发表时间：
1998-11-10
影响因子：
11.1
作者：
Dierich, A;Sairam, MR;Sassone-Corsi, P
通讯作者：
Sassone-Corsi, P
影响因子：
5.8
作者：
CAMPEN, CA;LAI, MT;RIVIER, J
通讯作者：
RIVIER, J
影响因子：
6.7
作者：
FRASER, HM
通讯作者：
FRASER, HM
影响因子：
3.7
作者：
Chakraborty P;Roy SK
通讯作者：
Roy SK