Global study of holistic morphological effectors in the budding yeast Saccharomyces cerevisiae.
Global study of holistic morphological effectors in the budding yeast Saccharomyces cerevisiae.
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DOI：
10.1186/s12864-018-4526-z
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发表时间：
2018-02-20
期刊：
影响因子：
4.4
通讯作者：
Ohya Y
中科院分区：
文献类型：
--
作者：
Suzuki G;Wang Y;Kubo K;Hirata E;Ohnuki S;Ohya Y
基因表型效应的大小已经在芽殖酵母Saccharomyces cerevisiae的适合度和特定形态性状方面进行了彻底的研究，但对总体形态异常知之甚少。我们确定了1126整体形态效应，导致严重的总体形态异常时删除，和2241特定的形态效应弱整体效应，但对酵母形态的独特影响。整体效应子属于许多基因功能类别，并作为网络枢纽，影响大量的形态性状，与大量的基因相互作用，并促进高蛋白质表达。整体形态学异常有助于估计基因对形态学的重要性。基因重要性对适应度和形态学的贡献可以用来有效地将基因分类到功能组中。整体形态异常可作为一种可重复的、可靠的基因特征用于高维形态表型分析。它可以用于许多功能基因组学应用。本文的在线版本（10.1186/s12864-018-4526-z）包含补充材料，可供授权用户使用。
The size of the phenotypic effect of a gene has been thoroughly investigated in terms of fitness and specific morphological traits in the budding yeast Saccharomyces cerevisiae, but little is known about gross morphological abnormalities. We identified 1126 holistic morphological effectors that cause severe gross morphological abnormality when deleted, and 2241 specific morphological effectors with weak holistic effects but distinctive effects on yeast morphology. Holistic effectors fell into many gene function categories and acted as network hubs, affecting a large number of morphological traits, interacting with a large number of genes, and facilitating high protein expression. Holistic morphological abnormality was useful for estimating the importance of a gene to morphology. The contribution of gene importance to fitness and morphology could be used to efficiently classify genes into functional groups. Holistic morphological abnormality can be used as a reproducible and reliable gene feature for high-dimensional morphological phenotyping. It can be used in many functional genomic applications. The online version of this article (10.1186/s12864-018-4526-z) contains supplementary material, which is available to authorized users.
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