Vanillin inhibits translation and induces messenger ribonucleoprotein (mRNP) granule formation in saccharomyces cerevisiae: application and validation of high-content, image-based profiling.
Vanillin inhibits translation and induces messenger ribonucleoprotein (mRNP) granule formation in saccharomyces cerevisiae: application and validation of high-content, image-based profiling.
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DOI：
10.1371/journal.pone.0061748
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ohya Y
中科院分区：
文献类型：
--
作者：
Iwaki A;Ohnuki S;Suga Y;Izawa S;Ohya Y
木质纤维素酸水解产生的香草醛是酿酒酵母生长的有效抑制剂。在这里，我们研究了香草醛影响的细胞过程，使用高含量，基于图像的分析。在4，718个非必需酵母缺失突变体中，核糖体大亚基缺陷的突变体的形态与香草醛处理的细胞的形态具有显著的相似性。这些突变体中的缺陷集中在核糖体的三个结构域：mRNA隧道入口、出口和小亚基附着所需的骨架。为了证实香草醛抑制核糖体功能，我们评估了香草醛处理后多核糖体和信使核糖核蛋白颗粒的形成。多核糖体谱的分析显示在香草醛存在下多核糖体解体。香草醛处理后，形成了由非翻译mRNA和各种蛋白质组成的加工体和应激颗粒。这些结果表明香草醛抑制酵母细胞中的翻译。
Vanillin, generated by acid hydrolysis of lignocellulose, acts as a potent inhibitor of the growth of the yeast Saccharomyces cerevisiae. Here, we investigated the cellular processes affected by vanillin using high-content, image-based profiling. Among 4,718 non-essential yeast deletion mutants, the morphology of those defective in the large ribosomal subunit showed significant similarity to that of vanillin-treated cells. The defects in these mutants were clustered in three domains of the ribosome: the mRNA tunnel entrance, exit and backbone required for small subunit attachment. To confirm that vanillin inhibited ribosomal function, we assessed polysome and messenger ribonucleoprotein granule formation after treatment with vanillin. Analysis of polysome profiles showed disassembly of the polysomes in the presence of vanillin. Processing bodies and stress granules, which are composed of non-translating mRNAs and various proteins, were formed after treatment with vanillin. These results suggest that vanillin represses translation in yeast cells.
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