PDGF receptor alpha+ mesoderm contributes to endothelial and hematopoietic cells in mice.
PDGF receptor alpha+ mesoderm contributes to endothelial and hematopoietic cells in mice.
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DOI：
10.1002/dvdy.23923
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发表时间：
2013-03
影响因子：
2.5
通讯作者：
Kataoka, Hiroshi
中科院分区：
文献类型：
--
作者：
Ding, Guo;Tanaka, Yosuke;Hayashi, Misato;Nishikawa, Shin-Ichi;Kataoka, Hiroshi
背景：早期中胚层可分为 Flk-1+ 或 PDGF 受体 α (PDGFRα)+ 群体，分别大致代表外侧中胚层和近轴中胚层。已证明所有内皮 (EC) 和造血 (HPC) 细胞均源自 Flk-1+ 细胞。尽管PDGFRα+细胞在体外ES分化中产生ECs/HPCs，但PDGFRα+群体是否可以成为血内皮谱系尚未在小鼠胚胎中得到证实。结果：使用 PDGFRαMerCreMer 小鼠，PDGFRα+ 早期中胚层被证明有助于形成内皮细胞，包括造血 EC、胎儿肝 B 淋巴细胞和 Lin-Kit+Sca-1+ (KSL) 细胞。 PDGFRα+中胚层对EC和HPC的贡献直到E8.5为止是有限的，表明直到E8.5为止存在的PDGFRα+/Flk-1+群体可能是来自PDGFRα+群体的血液内皮谱系的来源。 PDGFRα+ 细胞中 Etv2 的基因删除或 Runx1 的恢复证实了 PDGFRα+ 中胚层在血管发育和造血中的功能意义。 PDGFRα+ 细胞中 Etv2 缺失和 Runx1 恢复分别导致异常血管重塑和胎儿肝脏 CD45+ 和 Lin-Kit+Sca-1+ (KSL) 细胞的拯救。结论：内皮细胞和造血细胞可以来源于小鼠PDGFRα+早期中胚层。根据转基因胚胎的表型分析，PDGFRα+ 中胚层在血管发育和造血中具有重要功能。 Developmental Dynamics 242:254–268, 2013。© 2013 Wiley periodicals, Inc. PDGF 受体 α 阳性中胚层有助于生理性小鼠胚胎发生中的内皮细胞和造血细胞。早期胚胎中的PDGF受体α阳性中胚层与卵黄囊血岛中胚层不同，代表胚胎右侧造血细胞的来源。 PDGF 受体 α 阳性中胚层中 Etv2 或 Runx1 的基因操作证明了该中胚层子集在血管发育和造血中的功能意义。
Background: Early mesoderm can be classified into Flk-1+ or PDGF receptor alpha (PDGFRα)+ population, grossly representing lateral and paraxial mesoderm, respectively. It has been demonstrated that all endothelial (EC) and hematopoietic (HPC) cells are derived from Flk-1+ cells. Although PDGFRα+ cells give rise to ECs/HPCs in in vitro ES differentiation, whether PDGFRα+ population can become hemato-endothelial lineages has not been proved in mouse embryos. Results: Using PDGFRαMerCreMer mice, PDGFRα+ early mesoderm was shown to contribute to endothelial cells including hemogenic ECs, fetal liver B lymphocytes, and Lin-Kit+Sca-1+ (KSL) cells. Contribution of PDGFRα+ mesoderm into ECs and HPCs was limited until E8.5, indicating that PDGFRα+/Flk-1+ population that exists until E8.5 may be the source for hemato-endothelial lineages from PDGFRα+ population. The functional significance of PDGFRα+ mesoderm in vascular development and hematopoiesis was confirmed by genetic deletion of Etv2 or restoration of Runx1 in PDGFRα+ cells. Etv2 deletion and Runx1 restoration in PDGFRα+ cells resulted in abnormal vascular remodeling and rescue of fetal liver CD45+ and Lin-Kit+Sca-1+ (KSL) cells, respectively. Conclusions: Endothelial and hematopoietic cells can be derived from PDGFRα+ early mesoderm in mice. PDGFRα+ mesoderm is functionally significant in vascular development and hematopoiesis from phenotype analysis of genetically modified embryos. Developmental Dynamics 242:254–268, 2013. © 2013 Wiley Periodicals, Inc. PDGF receptor alpha–positive mesoderm contributes to endothelial and hematopoietic cells in physiological mouse embryogenesis. PDGF receptor alpha–positive mesoderm in early embryo is distinct from yolk sac blood island mesoderm, representing a source of hematopoietic cells on the embryo proper side. Genetic manipulation of Etv2 or Runx1 in PDGF receptor alpha–positive mesoderm demonstrates the functional significance of this mesoderm subset in vascular development and hematopoiesis.
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影响因子：
5.2
作者：
Wareing, Sarah;Mazan, Andrzej;Kouskoff, Valerie
通讯作者：
Kouskoff, Valerie
影响因子：
1.5
作者：
Samokhvalov, IM;Thomson, AM;Medvinsky, A
通讯作者：
Medvinsky, A
影响因子：
20.3
作者：
Drake, CJ;Fleming, PA
通讯作者：
Fleming, PA
DOI：
10.1073/pnas.0402270102
发表时间：
2005-01-04
影响因子：
11.1
作者：
Bertrand, JY;Giroux, S;Cumano, A
通讯作者：
Cumano, A
影响因子：
3.7
作者：
Nobuhisa, Ikuo;Yamasaki, Shoutarou;Taga, Tetsuya
通讯作者：
Taga, Tetsuya