Artificial Diiron Enzymes with a De Novo Designed Four-Helix Bundle Structure.
Artificial Diiron Enzymes with a De Novo Designed Four-Helix Bundle Structure.
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DOI：
10.1002/ejic.201500470
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发表时间：
2015-07
影响因子：
2.3
通讯作者：
Lombardi, Angela
中科院分区：
文献类型：
--
作者：
Chino, Marco;Maglio, Ornella;Nastri, Flavia;Pavone, Vincenzo;DeGrado, William F.;Lombardi, Angela
单个多肽链可以提供天文数字的构象异构体。大自然通过进化只选择了其中的一小部分，组成了一系列支架，可以提供支持生命所需的全套化学反应。这些结构模板是如此稳定，以至于它们允许几个突变而不破坏全局折叠，甚至具有结合几个外源辅因子的能力。从这个角度来看，金属辅因子在几个过程的调节和催化中起着至关重要的作用。大自然能够调节金属的化学性质，只采用几个配体和稍微不同的几何形状。几个支架和金属结合基序代表了文献中强烈兴趣的焦点。这篇综述讨论了广泛的四螺旋束折叠，通过作为一个支架的从头蛋白质设计的背景下，以获得生物传感或催化的基本生化成分的金属结合位点。特别是，我们描述了合理的改进的结构/功能的二铁氧蛋白模型，由于铁（DF）家庭。DF蛋白是由我们通过设计和严格表征的迭代过程开发的，这使得从结构模型转变为功能模型。本文报道的实施例证明了从头设计方法以及光谱和结构表征的协同应用对优化人工酶的催化性能的重要性。
A single polypeptide chain may provide an astronomical number of conformers. Nature selected only a trivial number of them through evolution, composing an alphabet of scaffolds, that can afford the complete set of chemical reactions needed to support life. These structural templates are so stable that they allow several mutations without disruption of the global folding, even having the ability to bind several exogenous cofactors. With this perspective, metal cofactors play a crucial role in the regulation and catalysis of several processes. Nature is able to modulate the chemistry of metals, adopting only a few ligands and slightly different geometries. Several scaffolds and metal-binding motifs are representing the focus of intense interest in the literature. This review discusses the widespread four-helix bundle fold, adopted as a scaffold for metal binding sites in the context of de novo protein design to obtain basic biochemical components for biosensing or catalysis. In particular, we describe the rational refinement of structure/function in diiron–oxo protein models from the due ferri (DF) family. The DF proteins were developed by us through an iterative process of design and rigorous characterization, which has allowed a shift from structural to functional models. The examples reported herein demonstrate the importance of the synergic application of de novo design methods as well as spectroscopic and structural characterization to optimize the catalytic performance of artificial enzymes.
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影响因子：
5.6
作者：
Calhoun, JR;Kono, H;Saven, JG
通讯作者：
Saven, JG
影响因子：
2.9
作者：
Calhoun, JR;Nastri, F;DeGrado, WF
通讯作者：
DeGrado, WF
DOI：
10.1039/j29690000823
发表时间：
1969-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY B-PHYSICAL ORGANIC
影响因子：
--
作者：
CORBETT, JF
通讯作者：
CORBETT, JF
影响因子：
6.8
作者：
BETZ, SF;RALEIGH, DP;DEGRADO, WF
通讯作者：
DEGRADO, WF
影响因子：
3.5
作者：
Chanco, Emmanuel;Choi, Yoo Seong;Zhao, Huimin
通讯作者：
Zhao, Huimin