Inducible aldehyde dehydrogenases from rat liver cytosol.
Inducible aldehyde dehydrogenases from rat liver cytosol.
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来自大鼠肝细胞质的诱导型乙醛脱氢酶。
DOI：
10.1016/0041-008x(85)90340-0
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发表时间：
1985
影响因子：
3.8
通讯作者：
Deitrich,RA
中科院分区：
文献类型：
--
作者：
Simpson,VJ;Baker,R;Deitrich,RA
用硫酸铵分级分离、离子交换和亲和层析从大鼠肝胞液中分离纯化了苯巴比妥诱导的醛脱氢酶同工酶φ和TCDD诱导的醛脱氢酶同工酶τ。在pH9.6时，以丙醛为底物，NAD为辅因子，φ和τ同工酶的比活力分别为2850和5250 nmol NADH/min/mg蛋白。从凝胶过滤色谱法得到的分子量估计值φ为118，000 Da，τ为106，000 Da。在电聚焦柱中测定τ酶的等电点为6.5，通过免疫电泳测定φ酶的等电点约为7.2。这两种酶可以氧化各种醛底物，Km值范围从毫摩尔到微摩尔。使用NAD作为辅因子的长链脂肪族和芳香族醛往往是最好利用的底物。只有τ酶能够使用NADP作为辅因子。在pH 7.2时，测得乙醛的Km为φ 1.97 mm，τ为12.1 mm。两种酶对亚砷酸钠和双硫仑显示出相似的抑制特性，尽管φ酶对所有抑制剂的敏感性略高。
Two rat hepatic cytoplasmic isozymes of aldehyde dehydrogenase, φ, induced by phenobarbital treatment, and τ, induced by TCDD treatment, have been purified from rat hepatic cytosol by ammonium sulfate fractionation, followed by ion-exchange and affinity chromatography. The specific activities of the two isozymes at pH 9.6 with propionaldehyde as substrate and NAD as cofactor were 2850 and 5250 nmol of NADH/min/mg protein for φ and τ isozymes, respectively. Estimates of molecular weights from gel filtration chromatography gave values of 118,000 Da for φ and 106,000 Da for τ. An isoelectric point for the τ enzyme of 6.5 was determined in an electrofocusing column, and approximately 7.2 for φ by immunoelectrophoresis. Both enzymes can oxidize a wide variety of aldehyde substrates, with Kmvalues ranging from millimolar to micromolar. Long-chain aliphatic and aromatic aldehydes using NAD as cofactor tend to be the best utilized substrates. Only the τ enzyme is able to use NADP as cofactor. The measured Kmfor φ at pH 7.2 for acetaldehyde was 1.97 mm and for τ, 12.1 mm. Both enzymes showed similar inhibition characteristics with sodium arsenite and disulfiram, although the φ enzyme tended to be slightly more sensitive to all inhibitors.
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分子筛色谱中的分子排除和限制扩散过程。
影响因子：
2.9
作者：
G. K. Ackers
通讯作者：
G. K. Ackers
苯巴比妥和四氯二苯并-对-二恶英诱导不同的大鼠肝上清液醛脱氢酶。
影响因子：
4.8
作者：
R. Deitrich;P. Bludeau;T. Stock;M. Roper
通讯作者：
M. Roper
影响因子：
5.8
作者：
R. Deitrich;P. Bludeau;M. Roper;J. Schmuck
通讯作者：
J. Schmuck
邻苯二甲醛对肺和肝细胞色素 P-450 的破坏。
影响因子：
3.8
作者：
J. Patel
通讯作者：
J. Patel
大鼠中苯巴比妥和致癌物诱导的醛脱氢酶的比较。
DOI：
--
发表时间：
1979
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
M. Marselos;R. Törrönen;T. Koivula;M. Koivusalo
通讯作者：
M. Koivusalo