Quantitation and mapping of tissue optical properties using modulated imaging.
Quantitation and mapping of tissue optical properties using modulated imaging.
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DOI：
10.1117/1.3088140
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发表时间：
2009-03
影响因子：
3.5
通讯作者：
Tromberg BJ
中科院分区：
文献类型：
--
作者：
Cuccia DJ;Bevilacqua F;Durkin AJ;Ayers FR;Tromberg BJ
我们描述了一种快速，非接触式成像方法，调制成像（MI），定量，宽场表征的光学吸收和散射特性的混浊介质的发展。MI利用频域采样和基于模型的空间调制传递函数（s-MTF）分析的原理。我们提出并比较解析扩散和概率蒙特卡罗漫反射模型在空间频率域。接下来，我们进行MI测量的组织模拟幻影表现出广泛的L* 值（0.5毫米至3毫米）和比率（8至500），报告的吸收和减少散射参数，分别约6%和3%的整体精度。只有两个空间频率的采样，实现了只有三个相机图像，被认为是足够的准确测定的光学特性。然后，我们在体内组织系统中进行MI测量，展示了人体前臂中吸收和散射光学对比度的空间映射以及静脉闭塞期间前臂的动态测量。最后，空间分辨率的指标进行评估，通过模拟和测量的空间异构幻影。
We describe the development of a rapid, noncontact imaging method, modulated imaging (MI), for quantitative, wide-field characterization of optical absorption and scattering properties of turbid media. MI utilizes principles of frequency-domain sampling and model-based analysis of the spatial modulation transfer function (s-MTF). We present and compare analytic diffusion and probabilistic Monte Carlo models of diffuse reflectance in the spatial frequency domain. Next, we perform MI measurements on tissue-simulating phantoms exhibiting a wide range of l* values (0.5 mm to 3 mm) and ratios (8 to 500), reporting an overall accuracy of approximately 6% and 3% in absorption and reduced scattering parameters, respectively. Sampling of only two spatial frequencies, achieved with only three camera images, is found to be sufficient for accurate determination of the optical properties. We then perform MI measurements in an in vivo tissue system, demonstrating spatial mapping of the absorption and scattering optical contrast in a human forearm and dynamic measurements of a forearm during venous occlusion. Last, metrics of spatial resolution are assessed through both simulations and measurements of spatially heterogeneous phantoms.
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DOI：
10.1364/josaa.4.000423
发表时间：
1987-03-01
影响因子：
1.9
作者：
BONNER, RF;NOSSAL, R;WEISS, GH
通讯作者：
WEISS, GH
DOI：
10.1073/pnas.91.11.4887
发表时间：
1994-05-24
影响因子：
11.1
作者：
BOAS, DA;OLEARY, MA;YODH, AG
通讯作者：
YODH, AG
影响因子：
1.3
作者：
MARQUET, P;BEVILACQUA, F;DEHALLER, EB
通讯作者：
DEHALLER, EB
影响因子：
2.9
作者：
ROTH, S;FREUND, I
通讯作者：
FREUND, I
DOI：
10.1364/josaa.11.002727
发表时间：
1994-10-01
影响因子：
1.9
作者：
HASKELL, RC;SVAASAND, LO;TROMBERG, BJ
通讯作者：
TROMBERG, BJ