Extracellular microvesicles that originated adipose tissue derived mesenchymal stem cells have the potential ability to improve rheumatoid arthritis on mice.
Extracellular microvesicles that originated adipose tissue derived mesenchymal stem cells have the potential ability to improve rheumatoid arthritis on mice.
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DOI：
10.1016/j.reth.2020.08.004
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发表时间：
2020-12
影响因子：
4.3
通讯作者：
Kuroda M
中科院分区：
文献类型：
--
作者：
Tsujimaru K;Takanashi M;Sudo K;Ishikawa A;Mineo S;Ueda S;Kumagai K;Kuroda M
间充质干细胞(MSCs)是一种很有前途的再生医学治疗工具。尤其是脂肪组织来源的间充质干细胞具有强大的治疗类风湿性关节炎的潜力，因为这些细胞可以控制免疫平衡。RA系统性地发生自身免疫性疾病。有趣的是，IL-1受体拮抗剂缺陷(IL-1ra−/−)小鼠可引起类风湿性关节炎等关节的炎症反应。在RA治疗中，虽然AMSC可以改善炎症活动，但细胞外微囊(EV)在RA治疗中的作用却鲜为人知。为了阐明MSC来源的EVS本身参与了RA的改善机制，我们检测了AMSC-EVS开发对RA的功能影响。我们从AMSCs的培养上清液中分离AMSCs来源的小鼠脂肪组织和纯化的EV。为了验证EVS是否能改善RA，我们用EVS或AMSCs给IL-1ra基因敲除小鼠作为RA模型小鼠。用免疫印迹法检测EVS包被因子，用ELISA法分析其对RA的改善作用。在本研究中，我们发现，野生型AMSC和AMSC-EVS组小鼠的关节肿胀程度比IL-1ra−/−小鼠-AMSC-EVS组和未治疗组小鼠的关节肿胀要小。我们在野生型小鼠AMSC-EVS中检测到IL-1ra的表达，而在IL-1ra−/−小鼠中未检测到。促炎症细胞因子在AMSC和AMSC-EVS中的表达变化明显，而在IL-1ra−/−小鼠中未见明显差异。在本研究中，我们得出结论：MSCs可能通过转移包括其MSC来源的EVS在内的IL-1ra等因子来改善RA。脂肪组织源性间充质干细胞(AMSC)S改善关节炎。AMSCs来源的细胞外小泡(EVS)本身就可以改善关节炎。AMSCs来源的EV含有IL-1受体拮抗剂。
Mesenchymal stem cells (MSCs) are promising therapeutic tools in regenerative medicine. In particularly adipose tissue derived MSC (AMSC) has powerful potential for the therapeutics of rheumatoid arthritis (RA) because these cells can control immune balance. RA systemically occurs autoimmune disease. Interestingly, IL-1 receptor antagonist deficient (IL-1ra−/−) mice induce inflammation in joints like RA. In RA therapy, although AMSC improves the inflammation activity, it is little known to play roles of extracellular microvesicles (EV) for improvement of RA. To clarify the MSC-derived EVs are involved amelioration mechanisms for RA by themselves, we examined the functional effects of development for RA by AMSC-EVs. We isolated AMSCs derived mice adipose tissue and purified EVs from the culture supernatant of AMSCs. To examine whether EVs can improve RA, we administrated EVs or AMSCs to IL-1ra knockout mice as RA model mice. We analyzed EVs-included factor by western blot methods and RA improvement effect by ELISA. In this study, we showed that the swellings of joints on mice in wild type AMSC and that in AMSC-EVs decreased than that in IL-1ra−/− mice-AMSC-EVs and in none-treated. We detected IL-1ra expression in AMSC-EVs in wild type mice but not that in IL-1ra−/− mice. Proinflammatory cytokine expression changes in mice showed in AMSCs and AMSC-EVs, but no apparent differences cytokine expressions were detected in IL-1ra−/− mice. In this study, we concluded that MSCs might improve RA by the transferring of factors such as IL-1ra, which are included their MSC derived- EVs. Adipose tissue derived MSC (AMSC)s improves arthritis. AMSCs derived Extracellular vesicles (EVs) alone improve arthritis. AMSCs derived EVs contain IL-1 receptor antagonist.
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将不完全重编程与小鼠胚胎癌细胞来源的多能干细胞的多能性提高联系起来
影响因子：
3.7
作者：
Chang G;Miao YL;Zhang Y;Liu S;Kou Z;Ding J;Chen DY;Sun QY;Gao S
通讯作者：
Gao S
DOI：
10.18632/aging.101355
发表时间：
2017-12-28
期刊：
Aging
影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
5.3
作者：
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通讯作者：
Chiesa, Roberto
影响因子：
20.3
作者：
Lee, OK;Kuo, TK;Chen, TH
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