miRNA genes of an invasive vector mosquito, Aedes albopictus.
miRNA genes of an invasive vector mosquito, Aedes albopictus.
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DOI：
10.1371/journal.pone.0067638
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tu Z
中科院分区：
文献类型：
--
作者：
Gu J;Hu W;Wu J;Zheng P;Chen M;James AA;Chen X;Tu Z
白纹伊蚊是登革热和基孔肯雅病毒的载体，是热带和温带环境中的一种强大的入侵物种。microRNA（miRNAs）调控基因表达和生物学过程，包括胚胎发育、先天免疫和感染。虽然在一些蚊子中已经发现了许多miRNA，但还没有全面的努力来表征它们来自单一物种的不同发育阶段。Ae.中miRNAs的系统分析白纹伊蚊将提高我们对其基本生物学的理解，并为预防病毒传播提供新的策略。每个样品从胚胎、幼虫、蛹、成年雄性、糖喂养和血喂养的成年雌性获得1000 - 1400万个Illumina测序读数。共鉴定了119个miRNA基因，其中15个为新发现的基因，代表了215个miRNA或miRNA星星（miRNA*）序列。新发现的miRNA基因中有11个、2个和2个分别对伊蚊、蚊亚科和蚊科具有特异性。许多miRNA主要在一个或两个发育阶段积累，并且在血液餐后显示丰度差异的大量miRNA可能在血液诱导的蚊子生物学中很重要。所有Ae的靶标的基因本体（GO）分析。白纹伊蚊miRNAs为研究其在蚊子中的功能提供了一个有用的起点。这项研究是第一个基于蚊子物种所有发育阶段的小RNA样本深度测序的miRNA系统分析。许多miRNA与特定的生理状态有关，最值得注意的是，血液喂养前后。谱系特异性miRNA的分布与蚊子的生殖发育一致，并且许多伊蚊特异性miRNA的存在可能反映了伊蚊属和库蚊属之间的分歧。
Aedes albopictus, a vector of Dengue and Chikungunya viruses, is a robust invasive species in both tropical and temperate environments. MicroRNAs (miRNAs) regulate gene expression and biological processes including embryonic development, innate immunity and infection. While a number of miRNAs have been discovered in some mosquitoes, no comprehensive effort has been made to characterize them from different developmental stages from a single species. Systematic analysis of miRNAs in Ae. albopictus will improve our understanding of its basic biology and inform novel strategies to prevent virus transmission. Between 10–14 million Illumina sequencing reads per sample were obtained from embryos, larvae, pupae, adult males, sugar-fed and blood-fed adult females. A total of 119 miRNA genes represented by 215 miRNA or miRNA star (miRNA*) sequences were identified, 15 of which are novel. Eleven, two, and two of the newly-discovered miRNA genes appear specific to Aedes, Culicinae, and Culicidae, respectively. A number of miRNAs accumulate predominantly in one or two developmental stages and the large number that showed differences in abundance following a blood meal likely are important in blood-induced mosquito biology. Gene Ontology (GO) analysis of the targets of all Ae. albopictus miRNAs provides a useful starting point for the study of their functions in mosquitoes. This study is the first systematic analysis of miRNAs based on deep-sequencing of small RNA samples of all developmental stages of a mosquito species. A number of miRNAs are related to specific physiological states, most notably, pre- and post-blood feeding. The distribution of lineage-specific miRNAs is consistent with mosquito phylogeny and the presence of a number of Aedes-specific miRNAs likely reflects the divergence between the Aedes and Culex genera.
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影响因子：
7
作者：
de Wit, Elzo;Linsen, Sam E. V.;Berezikov, Eugene
通讯作者：
Berezikov, Eugene
影响因子：
7
作者：
Berezikov, Eugene;Robine, Nicolas;Lai, Eric C.
通讯作者：
Lai, Eric C.
影响因子：
5.4
作者：
Leger, P.;Lara, E.;Bouloy, M.
通讯作者：
Bouloy, M.
DOI：
10.1139/g86-027
发表时间：
1986-04-01
期刊：
CANADIAN JOURNAL OF GENETICS AND CYTOLOGY
影响因子：
--
作者：
MARCHI, A;RAI, KS
通讯作者：
RAI, KS
影响因子：
56.9
作者：
Lim, LP;Glasner, ME;Bartel, DP
通讯作者：
Bartel, DP