Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cell.
Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cell.
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DOI：
10.1083/jcb.152.5.1079
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发表时间：
2001-03-05
期刊：
影响因子：
--
通讯作者：
Nomura R
中科院分区：
文献类型：
--
作者：
Fujimoto T;Kogo H;Ishiguro K;Tauchi K;Nomura R
小窝蛋白-1和小窝蛋白-2在非肌肉细胞中构成小窝的骨架。在本研究中，我们通过免疫荧光和免疫电子显微镜以及亚细胞分级，证明了小窝蛋白-2，特别是它的β亚型靶向于脂滴(LD)的表面。Brefeldin A处理诱导了小窝蛋白-2和小窝蛋白-1在LD中的进一步积累。对小鼠小窝蛋白-2缺失突变体的分析表明，中心疏水结构域(残基87-119)、NH2末端(残基70-86)和COOH末端(残基120-150)亲水结构域都是定位LD所必需的。NH2-和COOH-末端结构域似乎分别与膜结合和退出内质网有关，这意味着小窝蛋白-2是作为膜蛋白合成并运输到LD的。结合最近的发现，LD含有未酯化的胆固醇和RAFT蛋白，这一结果表明LD表面可能具有膜结构域的功能。这也表明LD与小窝蛋白介导的脂分子转运有关。
Caveolin-1 and -2 constitute a framework of caveolae in nonmuscle cells. In the present study, we showed that caveolin-2, especially its β isoform, is targeted to the surface of lipid droplets (LD) by immunofluorescence and immunoelectron microscopy, and by subcellular fractionation. Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD. Analysis of mouse caveolin-2 deletion mutants revealed that the central hydrophobic domain (residues 87–119) and the NH2-terminal (residues 70–86) and COOH-terminal (residues 120–150) hydrophilic domains are all necessary for the localization in LD. The NH2- and COOH-terminal domains appeared to be related to membrane binding and exit from ER, respectively, implying that caveolin-2 is synthesized and transported to LD as a membrane protein. In conjunction with recent findings that LD contain unesterified cholesterol and raft proteins, the result implies that the LD surface may function as a membrane domain. It also suggests that LD is related to trafficking of lipid molecules mediated by caveolins.
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影响因子：
4.8
作者：
Mora, R;Bonilha, VL;Rodriguez-Boulan, E
通讯作者：
Rodriguez-Boulan, E
DOI：
10.1083/jcb.131.6.1715
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nakamura N;Rabouille C;Watson R;Nilsson T;Hui N;Slusarewicz P;Kreis TE;Warren G
通讯作者：
Warren G
影响因子：
2.4
作者：
Kogo, H;Shioya, M;Fujimoto, T
通讯作者：
Fujimoto, T
影响因子：
4.8
作者：
Uittenbogaard, A;Ying, YS;Smart, EJ
通讯作者：
Smart, EJ
影响因子：
7.8
作者：
CONRAD, PA;SMART, EJ;YING, YS;ANDERSON, RGW;BLOOM, GS
通讯作者：
BLOOM, GS