Caveolin cycles between plasma membrane caveolae and the Golgi complex by microtubule-dependent and microtubule-independent steps.
Caveolin cycles between plasma membrane caveolae and the Golgi complex by microtubule-dependent and microtubule-independent steps.
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DOI：
10.1083/jcb.131.6.1421
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发表时间：
1995-12
影响因子：
7.8
通讯作者：
BLOOM, GS
中科院分区：
文献类型：
--
作者：
CONRAD, PA;SMART, EJ;YING, YS;ANDERSON, RGW;BLOOM, GS
小窝蛋白是一种与修饰质膜小窝细胞质表面的特征性外壳相关的蛋白质。最近发现，将人成纤维细胞暴露于胆固醇氧化酶（CO）快速诱导小窝蛋白重新分布到ER，然后再分布到高尔基复合体，并且随后去除CO允许小窝蛋白返回质膜（Smart，E. J.，Y.-- S. Ying，P. A.康拉德河G. W.安德森，细胞生物学杂志1994，127：1185-1197）。我们现在提出的证据表明，窖蛋白通常进行微管依赖性细胞质膜和高尔基体之间的循环。在用诺考达唑短暂处理，然后用诺考达唑加CO的混合物处理的细胞中，小窝蛋白从质膜重新定位到ER，然后到ER/高尔基体中间室（ERGIC），但没有观察到随后移动到高尔基体。即使在没有CO的情况下，诺考达唑也会导致小窝蛋白在ERGIC中积累。诺考达唑没有延迟的运动后，从高尔基体的质膜的小窝蛋白去除CO。在15度的细胞培养，然后升高的温度到37度导致小窝蛋白积累首先在ERGIC，然后在高尔基体，最后才重新建立其正常的稳态分布主要在质膜小窝。在释放15度阻滞的细胞中，nocodazole不抑制小窝蛋白从高尔基体到质膜的运动。综上所述，这些结果意味着小窝蛋白通过多步骤过程在质膜和高尔基体之间组成性循环，其中之一，ERGIC到高尔基体的运输需要微管。这种新的双向途径可能表明微管在维持小窝中的作用，以及小窝蛋白在质膜和ER/高尔基体系统之间穿梭脂肪酸和胆固醇的作用。
Caveolin is a protein associated with the characteristic coats that decorate the cytoplasmic face of plasma membrane caveolae. Recently it was found that exposure of human fibroblasts to cholesterol oxidase (CO) rapidly induces caveolin to redistribute to the ER and then to the Golgi complex, and that subsequent removal of CO allows caveolin to return to the plasma membrane (Smart, E. J., Y.-S. Ying, P. A. Conrad, R. G. W. Anderson, J. Cell Biol. 1994, 127:1185-1197). We now present evidence that caveolin normally undergoes microtubule-dependent cycling between the plasma membrane and the Golgi. In cells that were treated briefly with nocodazole and then with a mixture of nocodazole plus CO, caveolin relocated from the plasma membrane to the ER and then to the ER/Golgi intermediate compartment (ERGIC), but subsequent movement to the Golgi was not observed. Even in the absence of CO, nocodazole caused caveolin to accumulate in the ERGIC. Nocodazole did not retard the movement of caveolin from the Golgi to the plasma membrane after removal of CO. Incubation of cells at 15 degrees followed by elevation of the temperature to 37 degrees caused caveolin to accumulate first in the ERGIC and then in the Golgi, before finally reestablishing its normal steady state distribution predominantly in plasma membrane caveolae. In cells released from a 15 degrees block, movement of caveolin from the Golgi to the plasma membrane was not inhibited by nocodazole. Taken together, these results imply that caveolin cycles constitutively between the plasma membrane and the Golgi by a multi- step process, one of which, ERGIC-to-Golgi transport, requires microtubules. This novel, bidirectional pathway may indicate roles for microtubules in the maintenance of caveolae, and for caveolin in shuttling fatty acids and cholesterol between the plasma membrane and the ER/Golgi system.
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DOI：
10.1083/jcb.128.3.293
发表时间：
1995-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lippincott-Schwartz J;Cole NB;Marotta A;Conrad PA;Bloom GS
通讯作者：
Bloom GS
影响因子：
7.8
作者：
Parton, R G;Joggerst, B;Simons, K
通讯作者：
Simons, K
DOI：
10.1083/jcb.103.4.1557
发表时间：
1986-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Terasaki M;Chen LB;Fujiwara K
通讯作者：
Fujiwara K
DOI：
10.1083/jcb.37.3.633
发表时间：
1968-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Palade GE;Bruns RR
通讯作者：
Bruns RR
影响因子：
64.5
作者：
ROTHBERG, KG;HEUSER, JE;ANDERSON, RGW
通讯作者：
ANDERSON, RGW