Plasmodium vivax malaria serological exposure markers: Assessing the degree and implications of cross-reactivity with P. knowlesi.
Plasmodium vivax malaria serological exposure markers: Assessing the degree and implications of cross-reactivity with P. knowlesi.
复制标题
DOI：
10.1016/j.xcrm.2022.100662
复制
发表时间：
2022-06-21
影响因子：
14.3
通讯作者：
Mueller, Ivo
中科院分区：
文献类型：
--
作者：
Longley, Rhea J.;Grigg, Matthew J.;Schoffer, Kael;Obadia, Thomas;Hyslop, Stephanie;Piera, Kim A.;Nekkab, Narimane;Mazhari, Ramin;Takashima, Eizo;Tsuboi, Takafumi;Harbers, Matthias;Tetteh, Kevin;Drakeley, Chris;Chitnis, Chetan E.;Healer, Julie;Tham, Wai-Hong;Sattabongkot, Jetsumon;White, Michael T.;Cooper, Daniel J.;Rajahram, Giri S.;Barber, Bridget E.;William, Timothy;Anstey, Nicholas M.;Mueller, Ivo
血清学标记物是监测间日疟原虫疟疾和采取有针对性的干预措施的一个有前途的工具。间日疟原虫与人畜共患寄生虫诺氏疟原虫密切相关，诺氏疟原虫也感染人类。间日疟原虫和诺氏疟原虫在东南亚大部分地区共同流行，因此设计血清学标记物以最大限度地减少该地区的交叉反应非常重要。为了确定IgG对一组间日疟原虫血清学标志物的交叉反应性程度，我们测定了来自患有诺氏疟原虫疟疾的人类患者的样品。IgG抗体对间日疟原虫蛋白的反应性高，与它们的诺氏疟原虫直系同源物具有高序列同一性。IgG反应性在诺氏疟原虫感染后7天达到峰值，并且是短暂的，感染后1年的反应最小。我们设计了一组与诺氏疟原虫具有低水平交叉反应性的八种间日疟原虫蛋白。该面板可以准确地分类近期间日疟原虫感染，同时减少近期诺氏疟原虫感染的错误分类。血清学对于间日疟原虫疟疾的可操作监测是有希望的诺氏疟原虫感染诱导对间日疟原虫蛋白质的交叉反应性IgG抗体交叉反应性IgG抗体在感染后是短暂的。表征感染密切相关的寄生虫物种诺氏疟原虫的人中对间日疟原虫蛋白的交叉反应性。他们提出了一种方法，以保持间日疟原虫血清学暴露标志物的灵敏度，同时减少由于与诺氏疟原虫的交叉反应而导致的错误分类。
Serological markers are a promising tool for surveillance and targeted interventions for Plasmodium vivax malaria. P. vivax is closely related to the zoonotic parasite P. knowlesi, which also infects humans. P. vivax and P. knowlesi are co-endemic across much of South East Asia, making it important to design serological markers that minimize cross-reactivity in this region. To determine the degree of IgG cross-reactivity against a panel of P. vivax serological markers, we assayed samples from human patients with P. knowlesi malaria. IgG antibody reactivity is high against P. vivax proteins with high sequence identity with their P. knowlesi ortholog. IgG reactivity peaks at 7 days post-P. knowlesi infection and is short-lived, with minimal responses 1 year post-infection. We designed a panel of eight P. vivax proteins with low levels of cross-reactivity with P. knowlesi. This panel can accurately classify recent P. vivax infections while reducing misclassification of recent P. knowlesi infections. Serology is promising for actionable surveillance for Plasmodium vivax malaria P. knowlesi infections induce cross-reactive IgG antibodies to P. vivax proteins Cross-reactive IgG antibodies are short-lived following infection Misclassification due to cross-reactivity can be reduced through antigen selection Longley et al. characterize cross-reactivity against Plasmodium vivax proteins in people infected with the closely related parasite species Plasmodium knowlesi. They propose an approach to maintain sensitivity of P. vivax serological exposure markers while reducing misclassification due to cross-reactivity with P. knowlesi.
登录
查看更多内容
DOI：
10.1093/cid/ciq249
发表时间：
2011-03-01
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Douglas NM;Nosten F;Ashley EA;Phaiphun L;van Vugt M;Singhasivanon P;White NJ;Price RN
通讯作者：
Price RN
间日疟原虫流行病学的变化：传统和新型监测工具的见解。
影响因子：
15.8
作者：
Auburn S;Cheng Q;Marfurt J;Price RN
通讯作者：
Price RN
影响因子：
6.2
作者：
De SL;Ntumngia FB;Nicholas J;Adams JH
通讯作者：
Adams JH
影响因子：
3
作者：
Kale, Sonal;Yadav, Chander P.;Mallick, Prashant K.
通讯作者：
Mallick, Prashant K.
影响因子：
7.7
作者：
França CT;White MT;He WQ;Hostetler JB;Brewster J;Frato G;Malhotra I;Gruszczyk J;Huon C;Lin E;Kiniboro B;Yadava A;Siba P;Galinski MR;Healer J;Chitnis C;Cowman AF;Takashima E;Tsuboi T;Tham WH;Fairhurst RM;Rayner JC;King CL;Mueller I
通讯作者：
Mueller I