Comparison of external and internal approaches for transplantation of autologous retinal pigment epithelium.

Comparison of external and internal approaches for transplantation of autologous retinal pigment epithelium.
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自体视网膜色素上皮移植的外部和内部方法的比较。

DOI:
--
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发表时间:
1992
影响因子:
4.4
通讯作者:
C. Dorey
C. Dorey
中科院分区:
医学2区
文献类型:
--
作者:
S. Wongpichedchai;J. Weiter;P. Weber;C. Dorey

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在兔模型上证实了自体视网膜色素上皮(RPE)细胞从锯齿缘后端到后极部移植的可行性。比较了两种将移植细胞引入视网膜下腔的技术:内部(前玻璃体)和外部(后巩膜)穿透视网膜下间隙。在这两种方法中,从兔眼外周视网膜活检获得RPE细胞,培养,用荧光染料和~3H-胸腺嘧啶核苷标记,然后移植到同侧或对侧眼的后极。体外方法始终导致在Bruch膜上移植更多的细胞。内部技术更精确,因为它允许直接可视化移植的RPE的位置。自体RPE的移植是一种应该进一步探索的可能性。
The feasibility of autologous transplantation of retinal pigment epithelial (RPE) cells from just posterior to the ora serrata to the posterior pole was demonstrated in the rabbit model. Two techniques for introducing the transplanted cells were compared: an internal (anterior transvitreal) and an external (posterior transscleral) penetration to the subretinal space. In both approaches, RPE cells were obtained by biopsy from the peripheral retina of a rabbit eye, cultured, labeled with a fluorescent dye and 3H-thymidine, and transplanted to the posterior pole of the same or contralateral eye. The external approach consistently resulted in a greater number of transplanted cells on Bruch's membrane. The internal technique was more precise because it permitted direct visualization of the placement of the transplanted RPE. Transplantation of autologous RPE is a possibility that should be further pursued.
DOI: 10.1016/0014-4835(90)90003-d
发表时间: 1990-01-01
影响因子: 3.4
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