Removal of peripheral blood monocytes by phenylalanine methyl ester has no effect on the colony growth of hematopoietic progenitor cells.
Removal of peripheral blood monocytes by phenylalanine methyl ester has no effect on the colony growth of hematopoietic progenitor cells.
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苯丙氨酸甲酯去除外周血单核细胞对造血祖细胞的集落生长没有影响。
DOI：
10.1002/stem.5530070204
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Dooley,DC
中科院分区：
文献类型：
--
作者：
Law,P;Schwartz,GN;Alsop,T;Haiber,LM;Smith,DM;Dooley,DC
Purification of hematopoietic cells often includes an adherence step for the removal of monocytes. Recent studies have shown that some hematopoietic cells are adherent and that plastic adhesion might activate monocyte/macrophage for cytokine production. We investigated the possibility of using L-phenylalanine methyl ester (PME) as an alternative approach to monocyte removal, particularly for large-scale cell separations. Cell yield, extent of monocyte removal and cloning efficiency of colony-forming progenitor cells were compared to that produced by plastic adhesion. Starting with mononuclear cells (MNC) from adult peripheral blood, cell recovery after PME treatment was higher than that achieved with adherence and fewer monocytes were present. Cloning efficiency of the granulocyte-monocyte colony-forming progenitor cells (CFU-gm) was not affected by the PME treatment. PME treatment and plastic adhesion of low-density cord blood cells produced comparable cell recovery, monocyte depletion and cloning efficiencies for CFU-gm and burst-forming units for erythroid progenitor cells (BFU-e). The PME procedure was optimized for the purification of hematopoietic cells from large quantities (< 1 10′) of low density, T cell-depleted peripheral blood MNC containing 60% to 85% monocytes. Compared to two cycles of plastic adhesion, PME treatment permitted higher cell recovery (5.9% vs. 1.3%), lower monocyte contamination (4.5% vs. 10.7%) and substantial cost, time and labor savings without compromising CFU-gm growth.
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DOI：
10.1172/jci112730
发表时间：
1986
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Charley,M;Thiele,DL;Bennett,M;Lipsky,PE
通讯作者：
Lipsky,PE
影响因子：
2.7
作者：
TAYLOR, MJ;BANK, HL;BENTON, MJ
通讯作者：
BENTON, MJ
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DOI：
10.1111/j.1600-0609.1982.tb00524.x
发表时间：
1982
期刊：
Scandinavian journal of haematology
影响因子：
--
作者：
Verma,DS;Spitzer,G;Johnston,DA;Beran,M;Zander,AR;McCredie,KB
通讯作者：
McCredie,KB
人粒细胞/巨噬细胞和红系祖细胞的体外分化：重组人促红细胞生成素、G-CSF、GM-CSF 和 IL-3 在补充血清和缺乏血清的培养物中的影响的比较分析。
影响因子：
20.3
作者：
Migliaccio,G;Migliaccio,AR;Adamson,JW
通讯作者：
Adamson,JW
低剂量照射对妊娠小鼠造血的影响
影响因子：
6.5
作者：
S. Weinberg;Emmeline G. McCarthy;T. MacVittie;S. J. Baum
通讯作者：
S. J. Baum