PICKLE is a CHD subfamily II ATP-dependent chromatin remodeling factor.
PICKLE is a CHD subfamily II ATP-dependent chromatin remodeling factor.
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DOI：
10.1016/j.bbagrm.2012.10.011
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发表时间：
2013-02
影响因子：
4.7
通讯作者：
Ogas, Joe
中科院分区：
文献类型：
--
作者：
Ho, Kwok Ki;Zhang, Heng;Golden, Barbara L.;Ogas, Joe
关键词：
PICKLE在抑制调节拟南芥发育身份的基因方面发挥着关键作用。PICKLE编码推定的ATP依赖性染色质重塑，其表现出与动物CHD重塑子家族II成员的序列相似性，所述动物CHD重塑子家族II包括也限制发育调节因子表达的重塑子如CHD 3/Mi-2。而动物CHD 3重塑是促进组蛋白脱乙酰化的Mi-2/NuRD复合物的组分，PICKLE促进组蛋白H3赖氨酸27的三甲基化，表明其通过不同的表观遗传途径起作用。在这里，我们研究是否PICKLE也是一个多亚基复合物的成员，并表征重组PICKLE蛋白的生化特性。系统发育分析表明，植物中的PICKLE相关蛋白与动物CHD重塑蛋白亚家族II的成员有着共同的祖先。然而，PICKLE在植物中的生物化学特性表明，PICKLE主要以单体形式存在。重组PICKLE蛋白是一种ATP酶，可被ssDNA和单核细胞体刺激，并与裸DNA和单核细胞体结合。此外，重组PICKLE表现出ATP依赖性染色质重塑活性。这些研究表明，植物中的亚家族II CHD蛋白，如PICKLE，保留ATP依赖性染色质重塑活性，但通过不涉及普遍存在的Mi-2/NuRD复合物的机制起作用。
PICKLE plays a critical role in repression of genes that regulate development identity in Arabidopsis thaliana. PICKLE codes for a putative ATP-dependent chromatin remodeler that exhibits sequence similarity to members of subfamily II of animal CHD remodelers, which includes remodelers such as CHD3/Mi-2 that also restrict expression of developmental regulators. Whereas animal CHD3 remodelers are a component of the Mi-2/NuRD complex that promotes histone deacetylation, PICKLE promotes trimethylation of histone H3 lysine 27 suggesting that it acts via a distinct epigenetic pathway. Here, we examine whether PICKLE is also a member of a multisubunit complex and characterize the biochemical properties of recombinant PICKLE protein. Phylogenetic analysis indicates that PICKLE-related proteins in plants share a common ancestor with members of subfamily II of animal CHD remodelers. Biochemical characterization of PICKLE in planta, however, reveals that PICKLE primarily exists as a monomer. Recombinant PICKLE protein is an ATPase that is stimulated by ssDNA and mononucleosomes and binds to both naked DNA and mononucleosomes. Furthermore, recombinant PICKLE exhibits ATP-dependent chromatin remodeling activity. These studies demonstrate that subfamily II CHD proteins in plants, such as PICKLE, retain ATP-dependent chromatin remodeling activity but act through a mechanism that does not involve the ubiquitous Mi-2/NuRD complex.
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发表时间：
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