Platelet-derived growth factor and transforming growth factor-beta 1 differentially affect the synthesis of biglycan and decorin by monkey arterial smooth muscle cells.
Platelet-derived growth factor and transforming growth factor-beta 1 differentially affect the synthesis of biglycan and decorin by monkey arterial smooth muscle cells.
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血小板源性生长因子和转化生长因子-β1 对猴动脉平滑肌细胞双糖链蛋白聚糖和核心蛋白聚糖的合成有不同的影响。
DOI：
10.1161/01.atv.13.7.1026
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Wight,TN
中科院分区：
文献类型：
--
作者：
Schönherr,E;Järveläinen,HT;Kinsella,MG;Sandell,LJ;Wight,TN
血小板源性生长因子（PDGF）和转化生长因子-β 1（TGF-β 1）是两种对动脉平滑肌细胞（ASMC）增殖具有相反作用的生长调节肽，研究了它们对两种小的硫酸软骨素/硫酸皮肤素蛋白聚糖（CS/DS PG）（称为双糖链蛋白聚糖和核心蛋白聚糖）合成的影响。用PDGF或TGF-β 1处理静止ASMCs 24小时，使[35 S]硫酸盐掺入双糖链聚糖分别增加3.3倍和2.9倍，而[35 S]硫酸盐掺入核心蛋白聚糖没有显著影响。用TGF-β 1而不是PDGF治疗使与编码双糖链蛋白聚糖的互补DNA（cDNA）杂交的信使RNA（mRNA）转录物的稳态水平增加一倍以上。这两种生长因子对与核心蛋白聚糖cDNA杂交的mRNA转录物的稳态水平几乎没有影响。加入PDGF或TGF-β 1后12至18小时，[35 S]硫酸盐掺入双糖链糖胺聚糖（GAG）中达到最大值。PDGF和TGF-β 1都增加了双糖链蛋白聚糖和核心蛋白聚糖的分子大小。这种增加是两种PG的核心蛋白上取代的较长GAG链合成的结果。PDGF而不是TGF-β 1导致这些新合成的GAG链中6 '-与4'-硫酸化二糖的比率增加两倍以上。这些结果表明，PDGF和TGF-β 1对培养的猴ASMCs合成小CS/DS PG具有特异性但不同的作用。
Platelet-derived growth factor (PDGF) and transforming growth factor-beta 1 (TGF-beta 1), two growth-regulatory peptides with opposite effects on arterial smooth muscle cell (ASMC) proliferation, were examined for their influence on the synthesis of two small chondroitin sulfate/dermatan sulfate proteoglycans (CS/DS PGs) called biglycan and decorin. Quiescent ASMCs treated with either PDGF or TGF-beta 1 for 24 hours increased [35S]sulfate incorporation into biglycan 3.3- and 2.9-fold, respectively, whereas the incorporation of [35S]sulfate into decorin was not significantly affected. Treatment with TGF-beta 1 but not PDGF more than doubled the steady-state level of messenger RNA (mRNA) transcripts hybridizing to a complementary DNA (cDNA) encoding biglycan. Both growth factors had little or no effect on steady-state levels of mRNA transcripts hybridizing to a decorin cDNA. Incorporation of [35S]sulfate into biglycan glycosaminoglycan (GAG) was maximal by 12 to 18 hours after either PDGF or TGF-beta 1 addition. Both PDGF and TGF-beta 1 increased the molecular sizes of biglycan and decorin. This increase was a result of the synthesis of longer GAG chains substituted on the core proteins of both PGs. PDGF but not TGF-beta 1 led to an increase of more than twofold in the ratio of 6'- to 4'-sulfated disaccharides in these newly synthesized GAG chains. These results indicate that PDGF and TGF-beta 1 have specific but different effects on the synthesis of small CS/DS PGs by monkey ASMCs in culture.
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从成熟牛关节软骨中分离硫酸皮肤素蛋白多糖。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rosenberg,LC;Choi,HU;Tang,LH;Johnson,TL;Pal,S;Webber,C;Reiner,A;Poole,AR
通讯作者：
Poole,AR
灵长类动脉中的蛋白聚糖。 iii。在培养中由动脉平滑肌细胞合成的蛋白聚糖的表征。
DOI：
10.1083/jcb.96.1.167
发表时间：
1983-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Wight TN;Hascall VC
通讯作者：
Hascall VC
DOI：
10.1161/01.atv.9.1.1
发表时间：
1989
期刊：
Arteriosclerosis
影响因子：
--
作者：
Thomas N. Wight
通讯作者：
Thomas N. Wight
影响因子：
19.6
作者：
W. A. Border;Seiya Okuda;R. Languino;E. Ruoslahti
通讯作者：
W. A. Border;Seiya Okuda;R. Languino;E. Ruoslahti
从人主动脉内膜/中膜制备物中分离出的硫酸软骨素/硫酸皮肤素蛋白聚糖的纯化和 N 末端氨基酸序列。
影响因子：
4.1
作者：
G. Stöcker;H. Meyer;C. Wagener;H. Greiling
通讯作者：
H. Greiling