Screening preeclamptic cord plasma for proteins associated with decreased breast cancer susceptibility.
Screening preeclamptic cord plasma for proteins associated with decreased breast cancer susceptibility.
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DOI：
10.1016/j.gpb.2013.09.009
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发表时间：
2013-12
影响因子：
9.5
通讯作者：
Hsieh, Chung-Cheng
中科院分区：
文献类型：
--
作者：
Low, Hoi Pang;Tiwari, Ashutosh;Janjanam, Jagadeesh;Qiu, Li;Chang, Chien-I;Strohsnitter, William C.;Norwitz, Errol R.;Tam, Sun W.;Evans, James E.;Green, Karin M.;Paulo, Joao A.;Lambe, Mats;Hsieh, Chung-Cheng
先兆子痫是一种以高血压和蛋白尿为特征的妊娠并发症，已发现可降低女性后代患乳腺癌的风险。由于这种保护作用可能是由于在宫内生活期间暴露于先兆子痫特异性蛋白质，因此比较了先兆子痫和血压正常妊娠之间的脐带血血浆蛋白质组学谱。使用nanoACQUITY超高效液相色谱-质谱联用定量方法（具有高能采集模式E（NanoUPLC-MSE））对缺乏14种丰富蛋白质的脐带血浆样本进行蛋白质组学分析。鉴定出69个差异表达蛋白，其中15个和6个蛋白分别仅在先兆子痫和正常妊娠中检测到。此外，8种蛋白质（凝溶胶蛋白、补体C5、角蛋白I型细胞骨架10、色素上皮衍生因子、补体因子B、补体成分C7、血红蛋白γ亚基2和甲胎蛋白）的表达在先兆子痫中上调，与血压正常的妊娠相比，其倍数变化为2.0。先兆子痫脐带血血浆中甲胎蛋白的鉴定支持了该筛选的有效性，因为甲胎蛋白具有抗雌激素特性，并且先前已与先兆子痫以及降低乳腺癌风险有关。这项初步研究的结果可能为子痫前期和成年后乳腺癌易感性降低之间的机制联系提供新的见解。
Preeclampsia, a complication of pregnancy characterized by hypertension and proteinuria, has been found to reduce the subsequent risk for breast cancer in female offspring. As this protective effect could be due to exposure to preeclampsia-specific proteins during intrauterine life, the proteomic profiles of umbilical cord blood plasma between preeclamptic and normotensive pregnancies were compared. Umbilical cord plasma samples, depleted of 14 abundant proteins, were subjected to proteomic analysis using the quantitative method of nanoACQUITY ultra performance liquid chromatography–mass spectrometry with elevated energy mode of acquisitionE (NanoUPLC-MSE). Sixty-nine differentially expressed proteins were identified, of which 15 and 6 proteins were only detected in preeclamptic and normotensive pregnancies, respectively. Additionally, expression of 8 proteins (gelsolin, complement C5, keratin type I cytoskeletal 10, pigment epithelium-derived factor, complement factor B, complement component C7, hemoglobin subunit gamma-2 and alpha-fetoprotein) were up-regulated in preeclampsia with a fold change of ⩾2.0 when compared to normotensive pregnancies. The identification of alpha-fetoprotein in preeclamptic umbilical cord blood plasma supported the validity of this screen as alpha-fetoprotein has anti-estrogenic properties and has previously been linked to preeclampsia as well as a reduced breast cancer risk. The findings of this pilot study may provide new insights into the mechanistic link between preeclampsia and potentially reduced breast cancer susceptibility in adult life.
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影响因子：
11.5
作者：
de Sa, Sandra Valeria;Correa-Giannella, Maria Lucia;Giannella-Neto, Daniel
通讯作者：
Giannella-Neto, Daniel
影响因子：
10.3
作者：
Ekbom, A;Hsieh, CC;Trichopoulos, D
通讯作者：
Trichopoulos, D
影响因子：
3.4
作者：
Aydin, S.;Guzel, S. P.;Halifeoglu, I.
通讯作者：
Halifeoglu, I.
影响因子：
4.3
作者：
Catarino, Cristina;Rebelo, Irene;Santos-Silva, Alice
通讯作者：
Santos-Silva, Alice
影响因子：
5.4
作者：
Lambe, M;Trichopoulos, D;Wide, L
通讯作者：
Wide, L