Anion secretion by the inner medullary collecting duct. Evidence for involvement of the cystic fibrosis transmembrane conductance regulator.
Anion secretion by the inner medullary collecting duct. Evidence for involvement of the cystic fibrosis transmembrane conductance regulator.
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阴离子由内髓集合管分泌。
DOI：
10.1172/jci117709
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Stokes,JB
中科院分区：
文献类型：
--
作者：
Husted,RF;Volk,KA;Sigmund,RD;Stokes,JB
已确定终末肾集合管能够进行产电Na+吸收。本实验研究了其他主动离子运输过程中的原代培养的大鼠内髓集合管。当阿米洛利类似物benzamil抑制生电Na+吸收，cAMP激动剂刺激跨单层短路电流，这是不依赖于在顶端的溶液中的Na+的存在，但依赖于Cl-和HCO 3-的存在。该电流不被袢利尿剂布美他尼抑制，但被哇巴因（一种Na+/K+泵抑制剂）抑制。电流被阴离子转运抑制剂降低，其特征与囊性纤维化跨膜传导调节因子（CFATR）Cl-通道抑制剂相似。使用几种PCR策略，我们证明了预测的长度和序列同一性与大鼠CFTR的片段。使用全细胞膜片钳分析，我们证明了cAMP刺激的氯电流的CFTR的特性。结论：大鼠内髓集合管具有分泌阴离子的能力。CFTR Cl-通道很可能参与了这一过程。图片
It is well established that the terminal renal collecting duct is capable of electrogenic Na+ absorption. The present experiments examined other active ion transport processes in primary cultures of the rat inner medullary collecting duct. When the amiloride analogue benzamil inhibited electrogenic Na+ absorption, cAMP agonists stimulated a transmonolayer short circuit current that was not dependent on the presence of Na+ in the apical solution, but was dependent on the presence of Cl- and HCO3-. This current was not inhibited by the loop diuretic bumetanide, but was inhibited by ouabain, an inhibitor of the Na+/K+ pump. The current was reduced by anion transport inhibitors, with a profile similar to that seen for inhibitors of the cystic fibrosis transmembrane conductance regulator (CFATR) Cl- channel. Using several PCR strategies, we demonstrated fragments of the predicted lengths and sequence identity with the rat CFTR. Using whole-cell patch-clamp analysis, we demonstrated a cAMP-stimulated Cl- current with characteristics of the CFTR. We conclude that the rat inner medullary collecting duct has the capacity to secrete anions. It is highly likely that the CFTR Cl- channel is involved in this process.Images
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加压素和 cAMP 刺激 IMCD 细胞系中的电化学氯化物分泌。
DOI：
10.1152/ajprenal.1995.268.5.f854
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Kizer,NL;Vandorpe,D;Lewis,B;Bunting,B;Russell,J;Stanton,BA
通讯作者：
Stanton,BA
DOI：
10.1113/jphysiol.1990.sp018192
发表时间：
1990
期刊：
The Journal of physiology
影响因子：
--
作者：
Matsuda,JJ;Volk,KA;Shibata,EF
通讯作者：
Shibata,EF
前列腺素 E2 通过环磷酸腺苷依赖性途径激活主细胞中的顶端 Cl- 通道簇。
DOI：
10.1172/jci117037
发表时间：
1994
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Ling,BN;Kokko,KE;Eaton,DC
通讯作者：
Eaton,DC
DOI：
10.1073/pnas.86.15.6007
发表时间：
1989-08-01
影响因子：
11.1
作者：
MANGOOKARIM, R;UCHIC, M;GRANTHAM, JJ
通讯作者：
GRANTHAM, JJ
盐和水分泌到林格输注大鼠的髓质集合管中。
DOI：
10.1152/ajplegacy.1975.228.2.565
发表时间：
1975
期刊：
The American journal of physiology
影响因子：
--
作者：
H. Sonnenberg
通讯作者：
H. Sonnenberg