Novel chromosomal insertions of ISEcp1-bla(CTX-M-15) and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli.
Novel chromosomal insertions of ISEcp1-bla(CTX-M-15) and diverse antimicrobial resistance genes in Zambian clinical isolates of Enterobacter cloacae and Escherichia coli.
复制标题
在赞比亚临床分离的阴沟肠杆菌和大肠杆菌中发现新的染色体插入ISEcp1-bla（CTX-M-15）和多种耐药基因。
DOI：
10.1186/s13756-021-00941-8
复制
发表时间：
2021-05-10
影响因子：
5.5
通讯作者：
Higashi H
中科院分区：
文献类型：
--
作者：
Shawa M;Furuta Y;Mulenga G;Mubanga M;Mulenga E;Zorigt T;Kaile C;Simbotwe M;Paudel A;Hang'ombe B;Higashi H
超广谱β-内酰胺酶（ESBLs）的流行病学发生了巨大的变化，CTX-M型酶占主导地位。编码CTX-M型ESBLs的blaCTX-M基因通常存在于质粒上，但染色体定位越来越常见。鉴于携带blaCTX-M的菌株通常表现出多药耐药（MDR），因此研究染色体整合的blaCTX-M与其他抗菌药物耐药（AMR）基因的存在之间的相关性并鉴定其他相关遗传元件非常重要。使用MiSeq和MinION对来自赞比亚的共46株头孢噻肟耐药肠杆菌科临床分离株（1株阴沟肠杆菌、9株肺炎克雷伯菌和36株大肠埃希菌）进行全基因组测序（WGS）。通过重建几乎完整的基因组，blaCTX-M基因被归类为染色体或质粒携带。基于WGS的基因分型鉴定了58个AMR基因，包括4个blaCTX-M等位基因（即，blaCTX-M-14、blaCTX-M-15、blaCTX-M-27和blaCTX-M-55）。使用选定的表型和基因型特征的层次聚类表明blaCTX-M基因的克隆传播。在45株携带blaCTX-M基因的菌株中，有7株在其染色体上携带该基因。在一个E. cloxacin和3个E. coli菌株，染色体blaCTX-M-15定位于大于10 kb的插入片段上。这些插入的ISEcp 1在一端的边界，表现出高度的核苷酸序列同源性与以前报道的质粒，并携带多个AMR基因，对应于表型AMR配置文件。我们的研究揭示了ISEcp 1-blaCTX-M-15和多个AMR基因在大肠杆菌染色体插入上的共存。cloxacin和E.这表明ISEcp 1可能负责不同AMR基因从质粒到染色体的转座。这种插入在染色体中的稳定保留可能有助于这些肠杆菌科物种之间的MDR克隆的成功繁殖。在线版本包含补充材料，可通过10.1186/s13756-021-00941-8获取。
The epidemiology of extended-spectrum β-lactamases (ESBLs) has undergone dramatic changes, with CTX-M-type enzymes prevailing over other types. blaCTX-M genes, encoding CTX-M-type ESBLs, are usually found on plasmids, but chromosomal location is becoming common. Given that blaCTX-M-harboring strains often exhibit multidrug resistance (MDR), it is important to investigate the association between chromosomally integrated blaCTX-M and the presence of additional antimicrobial resistance (AMR) genes, and to identify other relevant genetic elements. A total of 46 clinical isolates of cefotaxime-resistant Enterobacteriaceae (1 Enterobacter cloacae, 9 Klebsiella pneumoniae, and 36 Escherichia coli) from Zambia were subjected to whole-genome sequencing (WGS) using MiSeq and MinION. By reconstructing nearly complete genomes, blaCTX-M genes were categorized as either chromosomal or plasmid-borne. WGS-based genotyping identified 58 AMR genes, including four blaCTX-M alleles (i.e., blaCTX-M-14, blaCTX-M-15, blaCTX-M-27, and blaCTX-M-55). Hierarchical clustering using selected phenotypic and genotypic characteristics suggested clonal dissemination of blaCTX-M genes. Out of 45 blaCTX-M gene-carrying strains, 7 harbored the gene in their chromosome. In one E. cloacae and three E. coli strains, chromosomal blaCTX-M-15 was located on insertions longer than 10 kb. These insertions were bounded by ISEcp1 at one end, exhibited a high degree of nucleotide sequence homology with previously reported plasmids, and carried multiple AMR genes that corresponded with phenotypic AMR profiles. Our study revealed the co-occurrence of ISEcp1-blaCTX-M-15 and multiple AMR genes on chromosomal insertions in E. cloacae and E. coli, suggesting that ISEcp1 may be responsible for the transposition of diverse AMR genes from plasmids to chromosomes. Stable retention of such insertions in chromosomes may facilitate the successful propagation of MDR clones among these Enterobacteriaceae species. The online version contains supplementary material available at 10.1186/s13756-021-00941-8.
登录
查看更多内容
影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
3.9
作者：
Goswami, Cosmika;Fox, Stephen;Evans, Thomas J.
通讯作者：
Evans, Thomas J.
影响因子：
5.7
作者：
Kalungia, Aubrey Chichonyi;Burger, Johanita;Simuwelu, Chimwemwe
通讯作者：
Simuwelu, Chimwemwe
DOI：
10.1093/bioinformatics/btq413
发表时间：
2010-09-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Guy L;Kultima JR;Andersson SG
通讯作者：
Andersson SG
影响因子：
3.9
作者：
Goswami C;Fox S;Holden M;Connor M;Leanord A;Evans TJ
通讯作者：
Evans TJ