Bacterial flagella hijack type IV pili proteins to control motility.
Bacterial flagella hijack type IV pili proteins to control motility.
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DOI：
10.1073/pnas.2317452121
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发表时间：
2024-01-23
影响因子：
11.1
通讯作者：
Ottemann, Karen M.
中科院分区：
文献类型：
--
作者：
Liu, Xiaolin;Tachiyama, Shoichi;Zhou, Xiaotian;Mathias, Rommel A.;Bonny, Sharmin Q.;Khan, Mohammad F.;Xin, Yue;Roujeinikova, Anna;Liu, Jun;Ottemann, Karen M.
细菌表面附属物、鞭毛和IV型皮利（TFP）都被报道可以感知表面，但其机制还不清楚。这些细胞器以不同的方式运作-鞭毛旋转和TFP伸展和收缩。我们使用冷冻电子断层扫描，细菌遗传学，显微镜和生物化学显示，鞭毛已经从TFP中吸收了一组蛋白质，在鞭毛马达周围形成了一个笼子。这一结果是出乎意料的，因为以前没有人知道这两个附件共享的蛋白质。除了运动性粘附、小菌落形成和生物膜构建之外，这些增选的蛋白质赋予鞭毛调节活动的能力。这项工作提出了一个令人惊讶的想法，即通过TFP或鞭毛检测和传递的表面信号共享保守特征。细菌鞭毛和IV型皮利（TFP）是通过不同机制实现运动性和机械感测的表面附属物。这些结构以前被认为没有共同的组成部分。在这里，我们报告，TFP和一些鞭毛共享蛋白PilO，PilN和PilM，我们确定为幽门螺杆菌鞭毛电机的一部分。H.缺乏PilO或PilN的pylori突变体在半固体琼脂中比野生型迁移得更好，因为它们继续游动而不是聚集成小菌落，模仿了TFP调节的表面反应。像他们的TFP同源物，鞭毛PilO/PilN异二聚体形成了一个外围笼包围鞭毛电机。这些结果表明，PilO和PilN在鞭毛和TFP中通过差异调节细菌遇到表面时的运动性和小菌落形成而起类似作用。
Bacterial surface appendages, flagella, and type IV pili (TFP) are both reported to sense surfaces, but the mechanisms are not well understood. These organelles operate in different ways—flagella rotate and TFP extend and retract. We used cryo-electron tomography, bacterial genetics, microscopy, and biochemistry to show that flagella have coopted a set of proteins from TFP to form a cage around the flagellar motor. This result was unanticipated, as no one previously knew of proteins shared by these two appendages. These coopted proteins give flagella the ability to regulate activities in addition to motility—adherence, microcolony formation, and biofilm building. The work suggests the surprising idea that surface signaling—detected and transmitted through TFP or flagella—shares conserved features. Bacterial flagella and type IV pili (TFP) are surface appendages that enable motility and mechanosensing through distinct mechanisms. These structures were previously thought to have no components in common. Here, we report that TFP and some flagella share proteins PilO, PilN, and PilM, which we identified as part of the Helicobacter pylori flagellar motor. H. pylori mutants lacking PilO or PilN migrated better than wild type in semisolid agar because they continued swimming rather than aggregated into microcolonies, mimicking the TFP-regulated surface response. Like their TFP homologs, flagellar PilO/PilN heterodimers formed a peripheral cage that encircled the flagellar motor. These results indicate that PilO and PilN act similarly in flagella and TFP by differentially regulating motility and microcolony formation when bacteria encounter surfaces.
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DOI：
10.1126/science.aad2001
发表时间：
2016-03-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chang YW;Rettberg LA;Treuner-Lange A;Iwasa J;Søgaard-Andersen L;Jensen GJ
通讯作者：
Jensen GJ
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
4.6
作者：
Leighton TL;Mok MC;Junop MS;Howell PL;Burrows LL
通讯作者：
Burrows LL
影响因子：
3.2
作者：
Friedrich, Carmen;Bulyha, Iryna;Sogaard-Andersen, Lotte
通讯作者：
Sogaard-Andersen, Lotte
影响因子：
5.6
作者：
Ayers, M.;Sampaleanu, L. M.;Burrows, L. L.
通讯作者：
Burrows, L. L.