A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood.
A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood.
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DOI：
10.1038/s41598-023-34108-9
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发表时间：
2023-05-09
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
没有检测B的检测方法。感染莱姆病个体血液中的伯氏抗原。在这里，我们提供了可以量化B的原理证明证据。使用基于便携式智能手机的荧光显微镜测量纸质微流控芯片上的免疫凝集反应，我们瞄准了B。BurgdorferiOspA开发了一个工作原型，并增加了两个抗原（OspC和VlsE）的例子，具有诊断价值的莱姆病阶段的歧视。使用广泛验证的OspA单克隆抗体（LA-2），OspA抗原的检测在1%血液中具有高达100 pg/mL的宽线性范围，检测限（LOD）为100 fg/mL（未稀释血液中= 10 pg/mL），比我们的目标10 ng/mL低1000倍。使用多克隆和单克隆抗体对另外两个靶标进行分析。OspC抗原在LOD 100 pg/mL（= 10 ng/mL未稀释血液）下检出，VlsE抗原在LOD 1-10 pg/mL（= 0.1-1 ng/mL未稀释血液）下检出。该方法是准确的，从样品到答案在20分钟内进行。当优化检测几个B。通过检测莱姆病抗原，该检测可以区分活动性感染和既往感染，并有助于在感染的最初几周诊断莱姆病，此时抗体的存在通常低于血清学方法检测的阈值。
There are no assays for detecting B. burgdorferi antigen in blood of infected Lyme disease individuals. Here, we provide proof-of-principle evidence that we can quantify B. burgdorferi antigen in spiked blood using a portable smartphone-based fluorescence microscope that measures immunoagglutination on a paper microfluidic chip. We targeted B. burgdorferi OspA to develop a working prototype and added examples of two antigens (OspC and VlsE) that have diagnostic value for discrimination of Lyme disease stage. Using an extensively validated monoclonal antibody to OspA (LA-2), detection of OspA antigen had a broad linear range up to 100 pg/mL in 1% blood and the limit of detection (LOD) was 100 fg/mL (= 10 pg/mL in undiluted blood), which was 1000 times lower than our target of 10 ng/mL. Analysis of the two other targets was done using polyclonal and monoclonal antibodies. OspC antigen was detected at LOD 100 pg/mL (= 10 ng/mL of undiluted blood) and VlsE antigen was detected at LOD 1–10 pg/mL (= 0.1–1 ng/mL of undiluted blood). The method is accurate and was performed in 20 min from sample to answer. When optimized for detecting several B. burgdorferi antigens, this assay may differentiate active from past infections and facilitate diagnosis of Lyme disease in the initial weeks of infection, when antibody presence is typically below the threshold to be detected by serologic methods.
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影响因子：
14.8
作者：
Chung, Soo;Breshears, Lane E.;Yoon, Jeong-Yeol
通讯作者：
Yoon, Jeong-Yeol
影响因子：
12.6
作者：
Akarapipad P;Kaarj K;Breshears LE;Sosnowski K;Baker J;Nguyen BT;Eades C;Uhrlaub JL;Quirk G;Nikolich-Žugich J;Worobey M;Yoon JY
通讯作者：
Yoon JY
影响因子：
3.7
作者：
Eshoo, Mark W.;Crowder, Christopher C.;Aucott, John N.
通讯作者：
Aucott, John N.
影响因子：
9.4
作者：
Lahey, Lauren J.;Panas, Michael W.;Robinson, William H.
通讯作者：
Robinson, William H.
影响因子：
3.1
作者：
FUNG, BP;MCHUGH, GL;STEERE, AC
通讯作者：
STEERE, AC