Synergistic effects of Cydia pomonella granulovirus GP37 on the infectivity of nucleopolyhedroviruses and the lethality of Bacillus thuringiensis
Synergistic effects of Cydia pomonella granulovirus GP37 on the infectivity of nucleopolyhedroviruses and the lethality of Bacillus thuringiensis
复制标题
苹果颗粒病毒GP37对核多角体病毒感染力和苏云金芽孢杆菌致死率的协同作用
DOI：
10.1007/s00705-011-1039-3
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发表时间：
2011-06
影响因子：
2.7
通讯作者：
Sun, Xiulian
中科院分区：
文献类型：
--
作者：
Liu, Xiangyang;Ma, Xiaohe;Lei, Chengfeng;Xiao, Yuzhou;Zhang, Zhongxin;Sun, Xiulian
杆状病毒病毒粒子穿透寄主昆虫的围食膜(PM)是启动一次有效的初次感染的关键。经常报道，杆状病毒的增强素通过降解PM的各种蛋白质成分而显著增强病毒感染。然而，并不是所有的杆状病毒都编码增强素。杆状病毒的GP37s与虫痘病毒中发现的协同因子褐藻蛋白有很高的氨基酸同源性。在本研究中，截短的pomonella颗粒病毒gp37在大肠杆菌中表达。表达的GP37能有效地与几丁质结合，结合主要发生在3h内。滴食性生物测定表明，GP37显著增强了核型多角体病毒(NPV)对甜菜夜蛾幼虫的感染力和对苏云金芽孢杆菌(Bt)的致死作用。这是第一次证明杆状病毒GP37可以增强NPV和Bt的感染。
To initiate an efficient primary infection, it is important for baculovirus virions to penetrate through the peritrophic membrane (PM) of the host insect. It is frequently reported that enhancins of baculoviruses significantly enhance viral infection by degrading the various protein components of PMs. However, not all baculoviruses encode enhancins. GP37s of baculoviruses share high amino acid identity with fusolins, synergistic factors found in entomopoxviruses. In this study, a truncated Cydia pomonella granulovirus GP37 was expressed in Escherichia coli. The expressed GP37 effectively bound to chitin, and binding occurred predominantly within 3 h. GP37 altered the protein profiles of Spodoptera exigua PMs, from which a 50-kDa protein was dissociated. Droplet-feeding bioassays indicated that GP37 significantly enhanced the infectivity of nucleopolyhedroviruses (NPVs) and the lethality of Bacillus thuringiensis (Bt) in S. exigua larvae. This is the first demonstration of the enhancement of NPVs and Bt infection by a baculovirus GP37.
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影响因子：
5
作者：
Zhaofei Li;Chongbi Li;Kai Yang;Lihua Wang;C. Yin;Yingxue Gong;Y. Pang
通讯作者：
Zhaofei Li;Chongbi Li;Kai Yang;Lihua Wang;C. Yin;Yingxue Gong;Y. Pang
影响因子：
5.4
作者：
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通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
2.7
作者：
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通讯作者：
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影响因子：
3.4
作者：
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通讯作者：
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