Non-invasive detection of a small number of bioluminescent cancer cells in vivo.-猫眼课题宝

DOI：

10.1371/journal.pone.0009364

发表时间：

2010-02-23

期刊：

PloS one

影响因子：

3.7

通讯作者：

中科院分区：

综合性期刊3区

文献类型：

作者：

Kim JB;Urban K;Cochran E;Lee S;Ang A;Rice B;Bata A;Campbell K;Coffee R;Gorodinsky A;Lu Z;Zhou H;Kishimoto TK;Lassota P

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826408/?report=classic

肿瘤的早期发现可以显著提高肿瘤治疗的效果。癌症成像中最常见的问题之一是在活体动物中可以非侵入性地检测到多少细胞。虽然许多因素限制了这种检测，但增加细胞的光发射是克服这些限制的最有效方法之一。在这里，我们描述了一种慢病毒载体的开发和利用，其中含有增强型萤火虫荧光素酶（luc2）基因。得到的小鼠乳腺肿瘤的单细胞克隆（4T1-luc2）显示出在10，000光子/秒/细胞范围内的稳定光发射。在某些情况下，插入nu/nu小鼠皮肤下的单个4T1-luc2细胞可以在某些情况下使用冷却的CCD相机非侵入性地检测。此外，我们发现，在这些小鼠中只需要很少的细胞就可以形成肿瘤，并且在细胞植入后可以立即监测肿瘤的进展。这些细胞中显著更高的荧光素酶活性使我们能够检测到同基因Balb/c和nu/nu小鼠中的微转移。

Early detection of tumors can significantly improve the outcome of tumor treatment. One of the most frequently asked questions in cancer imaging is how many cells can be detected non-invasively in a live animal. Although many factors limit such detection, increasing the light emission from cells is one of the most effective ways of overcoming these limitations. Here, we describe development and utilization of a lentiviral vector containing enhanced firefly luciferase (luc2) gene. The resulting single cell clones of the mouse mammary gland tumor (4T1-luc2) showed stable light emission in the range of 10,000 photons/sec/cell. In some cases individual 4T1-luc2 cells inserted under the skin of a nu/nu mouse could be detected non-invasively using a cooled CCD camera in some cases. In addition, we showed that only few cells are needed to develop tumors in these mice and tumor progression can be monitored right after the cells are implanted. Significantly higher luciferase activity in these cells allowed us to detect micrometastases in both, syngeneic Balb/c and nu/nu mice.