The multiple roles of phosphate in muscle fatigue.
The multiple roles of phosphate in muscle fatigue.
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DOI：
10.3389/fphys.2012.00463
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发表时间：
2012
影响因子：
4
通讯作者：
Trajanovska S
中科院分区：
文献类型：
--
作者：
Allen DG;Trajanovska S
肌肉疲劳是在剧烈活动期间观察到的肌肉性能下降。在剧烈活动期间，ATP消耗超过产生，并且细胞内代谢物中存在多种变化，这可能有助于横桥活性的变化。同样公认的是，通过动作电位变化或肌浆网（SR）释放的Ca 2+减少而导致的激活减少对疲劳有额外的贡献。在这篇综述中，我们专注于细胞内无机磷酸盐（Pi）的作用，其浓度可以迅速增加，从5-30 mM左右，在强烈的疲劳。从皮肤肌纤维的研究表明，这些变化大大损害肌原纤维的性能，虽然效果是强烈的温度依赖性。增加的Pi也可以导致减少的Ca 2+从SR释放，因此可能有助于减少激活。在最近的一项研究中，我们已经测量了血液灌注的哺乳动物制剂中的Pi和Ca 2+释放，并且该制剂的结果使我们能够测试Pi和Ca 2+变化的组合效应可能导致疲劳的程度。
Muscle fatigue is the decline in performance of muscles observed during periods of intense activity. ATP consumption exceeds production during intense activity and there are multiple changes in intracellular metabolites which may contribute to the changes in crossbridge activity. It is also well-established that a reduction in activation, either through action potential changes or reduction in Ca2+ release from the sarcoplasmic reticulum (SR), makes an additional contribution to fatigue. In this review we focus on the role of intracellular inorganic phosphate (Pi) whose concentration can increase rapidly from around 5–30 mM during intense fatigue. Studies from skinned muscle fibers show that these changes substantially impair myofibrillar performance although the effects are strongly temperature dependent. Increased Pi can also cause reduced Ca2+ release from the SR and may therefore contribute to the reduced activation. In a recent study, we have measured both Pi and Ca2+ release in a blood-perfused mammalian preparation and the results from this preparation allows us to test the extent to which the combined effects of Pi and Ca2+ changes may contribute to fatigue.
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