Laser Doppler Flowmetry in CA1 Sector of Hippocampus and Cortex After Transient Forebrain Ischemia in Gerbils
Laser Doppler Flowmetry in CA1 Sector of Hippocampus and Cortex After Transient Forebrain Ischemia in Gerbils
复制标题
沙鼠短暂性前脑缺血后海马和皮质 CA1 区的激光多普勒血流测量
作者：
T. Kuroiwa;P. Bonnekoh;K. Hossmann
背景和目的：短暂性脑缺血血流动力学反应的局部差异可能与选择性易损性的发生有关。这些差异在大脑的脆弱和非脆弱区域进行了研究。方法：5只沙土鼠双侧前脑缺血10min，用激光多普勒血流仪连续测定额叶皮质和海马CA1区的脑血流量。用压力传感器同时记录颈动脉压。结果：脑缺血后，大脑皮质和CA1区血流量分别降至基础值的11.8%和18.0%。血管阻断解除后，皮质血流量在7.5min(恢复时间)恢复到缺血前水平，12.4min(峰潜伏期)达到充血高峰(123.8%)，27.2min再次下降到缺血前水平。在CA1区，血流在2.1min恢复到缺血前水平，5.7min达到充血高峰(122.2%)，21.3min再次下降到缺血前水平。在皮层和CA1区，恢复时间和峰潜伏期与缺血时的残余血流量呈负相关。大脑皮层神经元未见损伤，但仅有14.6%的CA1神经元存活1周。结论：尽管脑缺血时血流量下降较轻，脑缺血后血流量恢复较快，但CA1区神经元仍有选择性损伤。这些发现强调了内源性因素而不是血流动力学因素在短暂性双侧前脑缺血后CA1神经元选择性易损性发病机制中的重要性。
Background and Purpose: Local differences in the hemodynamic response to transient ischemia could be involved in the development of selective vulnerability. These differences were studied in vulnerable and nonvulnerable regions of the brain. Methods: Five gerbils were subjected to 10 minutes of bilateral forebrain ischemia, and cerebral blood flow was measured continuously in the frontal cortex and CA1 sector of the hippocampus using laser Doppler flowmetry. Carotid artery pressure was recorded simultaneously with a pressure transducer. Results: After induction of ischemia, blood flow in the cortex and CA1 sector decreased to 11.8% and 18.0% of the baseline value, respectively. After release of the vascular occlusion, blood flow in the cortex returned to the preischemic level at 7.5 minutes (recovery time), reached the hyperemic peak (123.8%) at 12.4 minutes (peak latency), and again decreased to the preischemic level at 27.2 minutes. In the CA1 sector, blood flow returned to the preischemic level at 2.1 minutes, reached the hyperemic peak (122.2%) at 5.7 minutes, and decreased again to the preischemic level at 21.3 minutes. In both the cortex and CA1 sector, recovery time and peak latency correlated inversely with the amount of residual blood flow during ischemia. Histologically, cortical neurons were not injured but only 14.6% of CA1 neurons survived 1 week after ischemia. Conclusions: CA1 neurons were selectively injured despite the milder percentage decrease of blood flow during ischemia and the more prompt recovery of flow after ischemia. These findings stress the importance of intrinsic rather than hemodynamic factors in the pathogenesis of selective vulnerability of CA1 neurons after transient bilateral forebrain ischemia.
通过穿通路径损伤改善海马结构缺血性神经元损伤的证据。
影响因子：
1.9
作者：
Wieloch,T;Lindvall,O;Blomqvist,P;Gage,FH
通讯作者：
Gage,FH