New insight into foregut functions of xenobiotic detoxification in the cockroach Periplaneta americana
New insight into foregut functions of xenobiotic detoxification in the cockroach Periplaneta americana
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对美洲大蠊蟑螂外源物质解毒前肠功能的新见解
DOI：
10.1111/1744-7917.12486
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发表时间：
2018-12
期刊：
影响因子：
4
通讯作者：
Ze Wen Liu
中科院分区：
文献类型：
--
作者：
Jian Hua Zhang;Song Zhang;Yuan Xue Yang;Yi Xi Zhang;Ze Wen Liu
昆虫前肠的生理功能，特别是外源性解毒功能，由于其外观不重要和分子信息不充分，报道甚少。美洲大蠊是一种昆虫学模式生物，其结构特点为研究前肠组织的生理功能提供了很好的材料。对新烟碱类杀虫剂环xaprid处理过的美洲大蠊个体（对照）和昆虫的前肠组织进行Illumina测序，得到54 193 166个clean reads，并进一步组装成53 853个平均长度为366 bp的单基因。此外，我们还分析了参与外源解毒的单基因数量，主要包括70个细胞色素p450、12个谷胱甘肽S转移酶（GSTs）、7个羧酸酯酶（CarEs）和7个三磷酸腺苷结合盒（ABC）转运蛋白。与对照相比，施用环xaprid后，22个外源解毒基因的表达上调，主要包含18个p450、1个GST、2个CarEs和1个ABC三磷酸腺苷转运蛋白，表明氧化还原是环xaprid处理后的主要反应过程。通过定量实时聚合酶链反应分析，环xaprid治疗后，所选单基因（6个p450, 1个GST和1个CarE）的表达上调至少2倍，并且与转录组数据基本一致。与先前的美洲假单胞菌中肠转录组相比，外源解毒基因的表达丰度可能是导致前肠和中肠解毒功能差异的重要因素。综上所述，昆虫前肠在与外源解毒有关的生理过程中也起着重要的作用。
The physiological functions of insect foregut, especially in xenobiotic detoxification, are scarcely reported because of unimportance in appearance and insufficient molecular information. The cockroach Periplaneta americana, an entomological model organism, provides perfect material to study physiological functions of foregut tissue due to its architectural feature. Through Illumina sequencing of foregut tissue from P. americana individuals (control) or insects treated with cycloxaprid, as a novel neonicotinoid insecticide, 54 193 166 clean reads were obtained and further assembled into 53 853 unigenes with an average length of 366 bp. Furthermore, the number of unigenes involved in xenobiotic detoxification was analyzed, mainly including 70 cytochrome P450s, 12 glutathione S‐transferases (GSTs), seven carboxylesterases (CarEs) and seven adenosine triphosphate‐binding cassette (ABC) transporters. Compared to control, the expression of 22 xenobiotic detoxification unigenes was up‐regulated after cycloxaprid application, mainly containing 18 P450s, one GST, two CarEs and one ABC adenosine triphosphate transporter, indicating that the oxidation‐reduction was the major reactive process to cycloxaprid application. Through quantitative real‐time polymerase chain reaction analysis, the expression of selected unigenes (six P450s, one GST and one CarE) was up‐regulated at least two‐fold following cycloxaprid treatment, and was generally in agreement with transcriptome data. Compared to the previous midgut transcriptome of P. americana, it looks like the expressive abundance of the xenobiotic detoxification unigenes might be important factors to the detoxifying functional differences between foregut and midgut. In conclusion, insect foregut would also play important roles in the physiological processes related to xenobiotic detoxification.
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作者：
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通讯作者：
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