Multiplexed detection of respiratory pathogens with a portable analyzer in a "raw-sample-in and answer-out" manner.
Multiplexed detection of respiratory pathogens with a portable analyzer in a "raw-sample-in and answer-out" manner.
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使用便携式分析仪以“原始样本输入和应答输出”的方式多重检测呼吸道病原体
DOI：
10.1038/s41378-021-00321-7
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发表时间：
2021
影响因子：
7.9
通讯作者：
Cheng J
中科院分区：
文献类型：
--
作者：
Li N;Shen M;Liu J;Zhang L;Wang H;Xu Y;Cheng J
2019冠状病毒病（COVID-19）已经出现，迅速蔓延，并在全球范围内造成严重的发病率和死亡率。公共卫生迫切需要快速、敏感、特异和现场诊断检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染。本研究基于固相核酸提取和逆转录环介导等温扩增（RT-LAMP）技术，开发了一种完全集成的便携式SARS-CoV-2检测仪。拭子可直接插入试剂卡中，用于呼吸道病原体的多重检测，无需预先准备。整个检测过程，包括拭子冲洗、基于磁珠的核酸提取和8重实时RT-LAMP，可在80分钟内在试剂盒中自动进行。通过检测SARS-CoV-2假病毒和三种其他呼吸道病原体的存在，即，肺炎克雷伯菌、铜绿假单胞菌和嗜麦芽窄食单胞菌。两组引物（N基因和ORF 1ab基因）对SARS-CoV-2假病毒的检出限（LoD）均为2.5拷贝/μL，在800 μL拭子冲洗液中成功检出3种细菌，LoD为2.5菌落形成单位（CFU）/μL。因此，本研究中开发的分析仪具有以“原始样品输入和应答”方式现场快速检测SARS-CoV-2和其他呼吸道病原体的潜力。
Coronavirus disease 2019 (COVID-19) has emerged, rapidly spread and caused significant morbidity and mortality worldwide. There is an urgent public health need for rapid, sensitive, specific, and on-site diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In this study, a fully integrated and portable analyzer was developed to detect SARS-CoV-2 from swab samples based on solid-phase nucleic acid extraction and reverse transcription loop-mediated isothermal amplification (RT-LAMP). The swab can be directly inserted into a cassette for multiplexed detection of respiratory pathogens without pre-preparation. The overall detection process, including swab rinsing, magnetic bead-based nucleic acid extraction, and 8-plex real-time RT-LAMP, can be automatically performed in the cassette within 80 min. The functionality of the cassette was validated by detecting the presence of a SARS-CoV-2 pseudovirus and three other respiratory pathogens, i.e., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The limit of detection (LoD) for the SARS-CoV-2 pseudovirus was 2.5 copies/μL with both primer sets (N gene and ORF1ab gene), and the three bacterial species were successfully detected with an LoD of 2.5 colony-forming units (CFU)/μL in 800 μL of swab rinse. Thus, the analyzer developed in this study has the potential to rapidly detect SARS-CoV-2 and other respiratory pathogens on site in a “raw-sample-in and answer-out” manner.
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结核病诊断的进展：XPERT MTB/RIF分析和未来的预期测试。
DOI：
10.1016/s1473-3099(13)70008-2
发表时间：
2013-04
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Lawn SD;Mwaba P;Bates M;Piatek A;Alexander H;Marais BJ;Cuevas LE;McHugh TD;Zijenah L;Kapata N;Abubakar I;McNerney R;Hoelscher M;Memish ZA;Migliori GB;Kim P;Maeurer M;Schito M;Zumla A
通讯作者：
Zumla A
影响因子：
3.4
作者：
Nguyen, Trieu;Bang, Dang Duong;Wolff, Anders
通讯作者：
Wolff, Anders
影响因子：
8.4
作者：
Liu, Dongchen;Zhu, Yunzeng;Xu, Youchun
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Xu, Youchun
用于菌尿多重核酸检测的独立且完全集成的流体盒系统
影响因子：
6.1
作者：
Li, Nan;Lu, Ying;Xu, Youchun
通讯作者：
Xu, Youchun
DOI：
10.3390/bios8010017
发表时间：
2018-02-26
期刊：
Biosensors
影响因子：
--
作者：
Mauk MG;Song J;Liu C;Bau HH
通讯作者：
Bau HH