Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests.
Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests.
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DOI：
10.3390/bios8010017
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发表时间：
2018-02-26
期刊：
影响因子：
--
通讯作者：
Bau HH
中科院分区：
文献类型：
--
作者：
Mauk MG;Song J;Liu C;Bau HH
本文综述了基于微流体技术的分子诊断设备（核酸扩增试验，NAATs）在传染病检测中的设计和应用，重点介绍了资源有限环境下的最小仪器、即时护理（POC）检测。结合固相核酸提取的微流体盒（“芯片”）；等温酶核酸扩增；预先储存的石蜡包封的冻干试剂；描述了实时或端点光学检测。这些芯片可以与配套模块一起使用，通过沉淀过滤的组合效果将血浆从血液中分离出来。三种报告类型：荧光、比色染料和生物发光；并比较了一种基于扩散反应柱的终点检测新范式。演示了多路复用（并行放大和检测多个目标）。低成本的检测和附加功能（数据分析、控制、通信）可以通过带有该芯片的手机平台来实现。调查了其他人的一些相关和类似目的的方法。
Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, with emphasis on minimally instrumented, point-of-care (POC) tests for resource-limited settings. Microfluidic cartridges (‘chips’) that combine solid-phase nucleic acid extraction; isothermal enzymatic nucleic acid amplification; pre-stored, paraffin-encapsulated lyophilized reagents; and real-time or endpoint optical detection are described. These chips can be used with a companion module for separating plasma from blood through a combined sedimentation-filtration effect. Three reporter types: Fluorescence, colorimetric dyes, and bioluminescence; and a new paradigm for end-point detection based on a diffusion-reaction column are compared. Multiplexing (parallel amplification and detection of multiple targets) is demonstrated. Low-cost detection and added functionality (data analysis, control, communication) can be realized using a cellphone platform with the chip. Some related and similar-purposed approaches by others are surveyed.
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影响因子：
2.8
作者：
Ahmad, Farhan;Seyrig, Gregoire;Hashsham, Syed A.
通讯作者：
Hashsham, Syed A.
影响因子：
6.1
作者：
Au AK;Bhattacharjee N;Horowitz LF;Chang TC;Folch A
通讯作者：
Folch A
影响因子：
5.2
作者：
Chander Y;Koelbl J;Puckett J;Moser MJ;Klingele AJ;Liles MR;Carrias A;Mead DA;Schoenfeld TW
通讯作者：
Schoenfeld TW
影响因子：
6.1
作者：
Begolo S;Shen F;Ismagilov RF
通讯作者：
Ismagilov RF
影响因子：
3.5
作者：
Bond, Meaghan M.;Richards-Kortum, Rebecca R.
通讯作者：
Richards-Kortum, Rebecca R.