Melanosome transfer to keratinocyte in the chicken embryonic skin is mediated by vesicle release associated with Rho-regulated membrane blebbing.
Melanosome transfer to keratinocyte in the chicken embryonic skin is mediated by vesicle release associated with Rho-regulated membrane blebbing.
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DOI：
10.1038/srep38277
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发表时间：
2016-12-02
影响因子：
4.6
通讯作者：
Takahashi Y
中科院分区：
文献类型：
--
作者：
Tadokoro R;Murai H;Sakai KI;Okui T;Yokota Y;Takahashi Y
在黑色素沉着的过程中，黑色素细胞中合成的黑色素通过一种称为黑素体的颗粒转移到角质形成细胞。以前的研究，主要是使用分离的培养细胞，提出了几种不同的模型，解释如何实现黑素体转移。在这里，使用一种技术，在一个三维系统，模仿自然组织的黑色素细胞的质膜标记，我们已经可视化的黑色素细胞的质膜与绿色荧光蛋白在鸡胚皮肤。共聚焦延时显微镜显示，黑素体转移介导的，至少部分，由质膜产生的囊泡。出乎意料的是，囊泡的释放伴随着黑素细胞的膜起泡。包封黑素体的水泡被捏掉成为囊泡，这些含有黑素体的囊泡最终被邻近的角质形成细胞吞噬。对于膜起泡和囊泡释放，Rho小GTdR是必不可少的。我们进一步表明，膜囊泡介导的黑素体转移在皮肤色素沉着中发挥着重要作用。鉴于鸡羽间空间的皮肤色素沉着与人类的毛发间空间相似，我们的发现应该在美容医学中具有重要意义。
During skin pigmentation in amniotes, melanin synthesized in the melanocyte is transferred to keratinocytes by a particle called the melanosome. Previous studies, mostly using dissociated cultured cells, have proposed several different models that explain how the melanosome transfer is achieved. Here, using a technique that labels the plasma membrane of melanocytes within a three-dimensional system that mimics natural tissues, we have visualized the plasma membrane of melanocytes with EGFP in chicken embryonic skin. Confocal time-lapse microscopy reveals that the melanosome transfer is mediated, at least in part, by vesicles produced by plasma membrane. Unexpectedly, the vesicle release is accompanied by the membrane blebbing of melanocytes. Blebs that have encapsulated a melanosome are pinched off to become vesicles, and these melanosome-containing vesicles are finally engulfed by neighboring keratinocytes. For both the membrane blebbing and vesicle release, Rho small GTPase is essential. We further show that the membrane vesicle-mediated melanosome transfer plays a significant role in the skin pigmentation. Given that the skin pigmentation in inter-feather spaces in chickens is similar to that in inter-hair spaces of humans, our findings should have important consequences in cosmetic medicine.
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DOI：
10.1083/jcb.200802081
发表时间：
2008-06-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fackler OT;Grosse R
通讯作者：
Grosse R
影响因子：
5.3
作者：
Kozma, R;Sarner, S;Lim, L
通讯作者：
Lim, L
影响因子：
4.8
作者：
Müller, I;Klocke, A;Engelmann, B
通讯作者：
Engelmann, B
DOI：
10.1016/j.cub.2009.09.059
发表时间：
2009-12-01
期刊：
Current biology : CB
影响因子：
--
作者：
Muralidharan-Chari V;Clancy J;Plou C;Romao M;Chavrier P;Raposo G;D'Souza-Schorey C
通讯作者：
D'Souza-Schorey C
影响因子：
3.7
作者：
Kormos B;Belso N;Bebes A;Szabad G;Bacsa S;Széll M;Kemény L;Bata-Csörgo Z
通讯作者：
Bata-Csörgo Z