In vitro dedifferentiation of melanocytes from adult epidermis.
In vitro dedifferentiation of melanocytes from adult epidermis.
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DOI：
10.1371/journal.pone.0017197
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发表时间：
2011-02-23
期刊：
影响因子：
3.7
通讯作者：
Bata-Csörgo Z
中科院分区：
文献类型：
--
作者：
Kormos B;Belso N;Bebes A;Szabad G;Bacsa S;Széll M;Kemény L;Bata-Csörgo Z
在以前的工作中，我们描述了一种新的培养技术，使用霍乱毒素和不含pma的培养基（Mel-mix）从成人表皮中获得纯黑素细胞培养物。在Mel-mix培养基中培养的黑素细胞是双极性的，无色素和高度增殖。进一步对培养的黑素细胞进行表征，发现c-Kit和TRP-1消失，nestin表达诱导，表明黑素细胞在体外培养中去分化。霍乱毒素和PMA能够诱导细胞中c-Kit和TRP-1蛋白的表达，逆转去分化。UV-B照射的角质细胞上清液可诱导去分化黑素细胞中TRP-1 mRNA的表达，但UV-B直接照射细胞可进一步降低TRP-1 mRNA的表达。这些去分化的、容易获得的培养黑素细胞为研究黑素细胞分化和可能的转分化提供了很好的模型。由于黑色素细胞在Mel-mix培养基中可以用人血清作为唯一补充进行培养，因此该培养体系也适用于自体细胞移植。
In previous work we described a novel culture technique using a cholera toxin and PMA-free medium (Mel-mix) for obtaining pure melanocyte cultures from human adult epidermis. In Mel-mix medium the cultured melanocytes are bipolar, unpigmented and highly proliferative. Further characterization of the cultured melanocytes revealed the disappearance of c-Kit and TRP-1 and induction of nestin expression, indicating that melanocytes dedifferentiated in this in vitro culture. Cholera toxin and PMA were able to induce c-Kit and TRP-1 protein expressions in the cells, reversing dedifferentiation. TRP-1 mRNA expression was induced in dedifferentiated melanocytes by UV-B irradiated keratinocyte supernatants, however direct UV-B irradiation of the cells resulted in further decrease of TRP-1 mRNA expression. These dedifferentiated, easily accessible cultured melanocytes provide a good model for studying melanocyte differentiation and possibly transdifferentiation. Because melanocytes in Mel-mix medium can be cultured with human serum as the only supplement, this culture system is also suitable for autologous cell transplantation.
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影响因子：
6.5
作者：
LERNER, AB;HALABAN, R;MOELLMANN, GE
通讯作者：
MOELLMANN, GE
影响因子：
6.5
作者：
Flammiger, Anna;Besch, Robert;Berking, Carola
通讯作者：
Berking, Carola
影响因子：
64.5
作者：
Adameyko, Igor;Lallemend, Francois;Ernfors, Patrik
通讯作者：
Ernfors, Patrik
DOI：
10.1073/pnas.97.14.7882
发表时间：
2000-07-05
影响因子：
11.1
作者：
Dupin, E;Glavieux, C;Le Douarin, NM
通讯作者：
Le Douarin, NM
DOI：
10.1083/jcb.200410115
发表时间：
2005-01-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Loercher AE;Tank EM;Delston RB;Harbour JW
通讯作者：
Harbour JW