Localization of a G-protein-coupled inwardly rectifying K+ channel, CIR, in the rat brain
Localization of a G-protein-coupled inwardly rectifying K+ channel, CIR, in the rat brain
复制标题
G 蛋白偶联内向整流 K 通道 (CIR) 在大鼠大脑中的定位
DOI：
10.1016/s0306-4522(96)00460-5
复制
发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
T. Kono
中科院分区：
文献类型：
--
作者：
Masaki Iizuka;I. Tsunenari;Y. Momota;I. Akiba;T. Kono
大鼠脑内G蛋白偶联K+通道CIR的细胞定位已被用CIR特异性抗体结合原位杂交证实。CIR蛋白和信使RNA分布于小脑皮质、海马结构、嗅觉系统、大脑皮层、基底节、下脑干多个核团和脉络丛。与集中在胞体细胞中的信使RNA不同，CIR蛋白在神经纤维的子集中发现，在其他情况下，在轴突终末中发现。在小脑皮质和海马区，CIR蛋白集中在篮状细胞的轴突末梢，已知为GABA能中间神经元。在黑质、下脑干的脚间核、三叉神经、舌下核、动眼神经核和红核，以及嗅球的簇状细胞和二尖瓣细胞中观察到蛋白质和信使RNA分布的差异。这些观察结果表明，CIR蛋白在细胞体合成后移位到神经纤维中。此外，其特殊的神经元定位，特别是在GABA能中间神经元，提示了CIR在神经元系统突触传递中的重要性。
The cellular localization of a G-protein-coupled K+channel, CIR, in the rat brain has been demonstrated using a CIR-specific antibody, in combination with in situ hybridization. The CIR protein and messenger RNA were found in the cerebellar cortex, hippocampal formation, olfactory system, cerebral cortex, basal ganglia, several nuclei of the lower brain stem and the choroid plexus. In contrast to the messenger RNA, which was concentrated in the cell soma, the CIR protein was found in a subset of nerve fibers and, in other cases, in axon terminals. In the cerebellar cortex and hippocampus, the CIR protein was concentrated in the axon terminals of basket cells which are known to be GABAergic interneurons. This discrepancy between the distribution of protein and messenger RNA was observed in the substantia nigra, the interpeduncular, trigeminal, hypoglossal, oculomotor and red nuclei of the lower brain stem, and the tufted and mitral cells of the olfactory bulb. These observations suggested the translocation of the CIR protein into the nerve fibers following synthesis in the cell soma. Furthermore, its specific neuronal localization, especially in GABAergic interneurons, suggested the importance of CIR in synaptic transmission in neuronal systems.
DOI：
10.1073/pnas.90.21.10235
发表时间：
1993-11-01
影响因子：
11.1
作者：
DASCAL, N;SCHREIBMAYER, W;DAVIDSON, N
通讯作者：
DAVIDSON, N
哺乳动物神经元记录的抑制性突触电位通过阻断去甲肾上腺素的摄取而延长。
DOI：
10.1113/jphysiol.1987.sp016357
发表时间：
1987
期刊：
The Journal of physiology
影响因子：
--
作者：
Surprenant,A;Williams,JT
通讯作者：
Williams,JT
新发现的脑钾通道由鸟嘌呤核苷酸结合蛋白 Go 门控。
DOI：
10.1126/science.3144040
发表时间：
1988
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
VanDongen,AM;Codina,J;Olate,J;Mattera,R;Joho,R;Birnbaumer,L;Brown,AM
通讯作者：
Brown,AM